Unsatisfactory biologic behavior of *I-fibrinogen labeled by the chloramine-T method

Judith M. Metzger, Roger H. Secker-Walker, Kenneth Krohn, Michael J. Welch, E. James Potchen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The blood clearance of canine fibrinogen labeled with radioactive iodine by the chloramine-T method has been examined in dogs, and the distribution of *I studied with a gamma scintillation camera. The labeled fibrinogen prepared by this method is more than 85 per cent clottable, but less than 20 per cent of the label remains attached to the fibrinogen to trace its behavior in vivo. Some of the administered material, in the form of aggregated fibrinogen, is rapidly removed from the circulation by the liver and spleen. A considerable proportion of the label is released as free iodide and is taken up by the thyroid gland, stomach, and salivary glands and is also excreted in the urine. When fibrinogen is labeled by the iodine monochloride method, between 2 and 5 times as much label remains attached to the fibrinogen to trace its fate. These findings should discourage the use of the chloramine-T method of labeling fibrinogen.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume82
Issue number2
StatePublished - 1973
Externally publishedYes

Fingerprint

Fibrinogen
Labels
Gamma Cameras
Liver Circulation
Scintillation
Iodides
chloramine-T
Salivary Glands
Iodine
Liver
Labeling
Canidae
Stomach
Thyroid Gland
Blood
Spleen
Cameras
Urine
Dogs

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Metzger, J. M., Secker-Walker, R. H., Krohn, K., Welch, M. J., & Potchen, E. J. (1973). Unsatisfactory biologic behavior of *I-fibrinogen labeled by the chloramine-T method. The Journal of Laboratory and Clinical Medicine, 82(2), 267-275.

Unsatisfactory biologic behavior of *I-fibrinogen labeled by the chloramine-T method. / Metzger, Judith M.; Secker-Walker, Roger H.; Krohn, Kenneth; Welch, Michael J.; Potchen, E. James.

In: The Journal of Laboratory and Clinical Medicine, Vol. 82, No. 2, 1973, p. 267-275.

Research output: Contribution to journalArticle

Metzger, JM, Secker-Walker, RH, Krohn, K, Welch, MJ & Potchen, EJ 1973, 'Unsatisfactory biologic behavior of *I-fibrinogen labeled by the chloramine-T method', The Journal of Laboratory and Clinical Medicine, vol. 82, no. 2, pp. 267-275.
Metzger, Judith M. ; Secker-Walker, Roger H. ; Krohn, Kenneth ; Welch, Michael J. ; Potchen, E. James. / Unsatisfactory biologic behavior of *I-fibrinogen labeled by the chloramine-T method. In: The Journal of Laboratory and Clinical Medicine. 1973 ; Vol. 82, No. 2. pp. 267-275.
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