Unidirectional transport of glucose and lactate into brain of fetal sheep and guinea-pig.

J. M. Bissonnette, Alan (Roger) Hohimer, C. R. Chao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The first-passage multiple-indicator dilution method was used to measure blood to brain transport of D- and L-glucose, D- and L-lactate and sucrose relative to 22Na, an impermeable reference tracer, in fetal sheep. Fractional extraction for D-glucose was 0.315 +/- 0.051 (S.E.M.) at normal glucose levels and fell to 0.198 +/- 0.041 at 5.2 +/- 0.4 mM-glucose. Fractional extractions for L-glucose, D- and L-lactate and sucrose were not different from zero. No specific blood-brain transport system was detected for L-lactate in fetal sheep in vivo (fractional extraction = -0.024 +/- 0.019). Uptake of L-lactate into isolated microvessels from fetal sheep cerebrum in vitro showed a slightly higher rate (32.2 +/- 8.9 pmol min-1 (mg protein)-1) than that for D-lactate (22.6 +/- 5.6). In fetal guinea-pigs, the carotid arterial injection method with tritiated water as the permeable reference was used to measure the brain uptake index (BUI). BUI was determined for D-glucose (0.304 +/- 0.065) sucrose (0.008 +/- 0.001), L-lactate (0.418 +/- 0.112) and D-lactate (0.071 +/- 0.024). Unidirectional influx calculated from these measurements and estimates of cerebral blood flow showed that transport would be rate-limiting for cerebral glucose utilization at arterial glucose levels below 0.5 mM in fetal sheep and 1.7 mM in fetal guinea-pig. In fetal sheep, but not in fetal guinea-pigs, lactate efflux may be limited by brain-blood transport.

Original languageEnglish (US)
Pages (from-to)515-523
Number of pages9
JournalExperimental Physiology
Volume76
Issue number4
StatePublished - Jul 1991
Externally publishedYes

Fingerprint

Lactic Acid
Sheep
Guinea Pigs
Glucose
Brain
Sucrose
Cerebrovascular Circulation
Cerebrum
Microvessels
Injections
Water
Proteins

ASJC Scopus subject areas

  • Physiology

Cite this

Bissonnette, J. M., Hohimer, A. R., & Chao, C. R. (1991). Unidirectional transport of glucose and lactate into brain of fetal sheep and guinea-pig. Experimental Physiology, 76(4), 515-523.

Unidirectional transport of glucose and lactate into brain of fetal sheep and guinea-pig. / Bissonnette, J. M.; Hohimer, Alan (Roger); Chao, C. R.

In: Experimental Physiology, Vol. 76, No. 4, 07.1991, p. 515-523.

Research output: Contribution to journalArticle

Bissonnette, JM, Hohimer, AR & Chao, CR 1991, 'Unidirectional transport of glucose and lactate into brain of fetal sheep and guinea-pig.', Experimental Physiology, vol. 76, no. 4, pp. 515-523.
Bissonnette, J. M. ; Hohimer, Alan (Roger) ; Chao, C. R. / Unidirectional transport of glucose and lactate into brain of fetal sheep and guinea-pig. In: Experimental Physiology. 1991 ; Vol. 76, No. 4. pp. 515-523.
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