Ultrastructural, morphometric, and immunocytochemical study of anterior hom cells in mice with “wasted” mutation

Helmi Lutsep, Moses Rodriguez

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mice with the autosomal recessive gene “wasted” (wst/wst) manifest hindlimb paralysis and tremulousness, develop reduced secretory immune responses, and have abnormal DNA repair mechanisms. There is prominent vacuolar degeneration of neurons within anterior horns of the spinal cord and motor nuclei of the brainstem. A morphometric analysis of motor neurons in the spinal cord was performed on 2-hydroxyethyl methacrylate-embedded tissue from ten wst/wst mice, ten littermates (wst/+, +/+) without clinical deficits, and ten parental (+/+) control mice. Vacuolated neurons were present only in wst/wst mice (p=0.0008). Fibrillary neurons were more numerous in the wst/wst mice than in littermates (p=0.01) or controls (p=0.007). The number of total or normal neurons did not differ significantly among the three groups. Volume measurements for normal, fibrillary, vacuolated, and total neurons were greater in wst/wst mice (p

Original languageEnglish (US)
Pages (from-to)519-533
Number of pages15
JournalJournal of Neuropathology and Experimental Neurology
Volume48
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Mutation
Neurons
Anterior Horn Cells
Recessive Genes
Motor Neurons
Hindlimb
Paralysis
DNA Repair
Brain Stem
Spinal Cord

Keywords

  • Amyotropic lateral sclerosis
  • Anterior horn
  • Morphometry
  • Motor neurons
  • Mouse mutant, “Wasted”
  • Neurofilaments

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Cellular and Molecular Neuroscience
  • Neurology
  • Neuroscience(all)

Cite this

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