Ultrasound-mediated vascular gene transfection by cavitation of endothelial-targeted cationic microbubbles

Aris Xie, Todd Belcik, Qi Yue, Terry Morgan, Shivam A. Champaneri, Sarah Taylor, Brian P. Davidson, Yan Zhao, Alexander L. Klibanov, Michael A. Kuliszewski, Howard Leong-Poi, Azzdine Ammi, Jonathan Lindner

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Objectives: Ultrasound-mediated gene delivery can be amplified by acoustic disruption of microbubble carriers that undergo cavitation. We hypothesized that endothelial targeting of microbubbles bearing cDNA is feasible and, through optimizing proximity to the vessel wall, increases the efficacy of gene transfection. Background: Contrast ultrasound-mediated gene delivery is a promising approach for site-specific gene therapy, although there are concerns with the reproducibility of this technique and the safety when using high-power ultrasound. Methods: Cationic lipid-shelled decafluorobutane microbubbles bearing a targeting moiety were prepared and compared with nontargeted microbubbles. Microbubble targeting efficiency to endothelial adhesion molecules (P-selectin or intercellular adhesion molecule [ICAM]-1) was tested using in vitro flow chamber studies, intravital microscopy of tumor necrosis factor-alpha (TNF-A)-stimulated murine cremaster muscle, and targeted contrast ultrasound imaging of P-selectin in a model of murine limb ischemia. Ultrasound-mediated transfection of luciferase reporter plasmid charge coupled to microbubbles in the post-ischemic hindlimb muscle was assessed by in vivo optical imaging. Results: Charge coupling of cDNA to the microbubble surface was not influenced by the presence of targeting ligand, and did not alter the cavitation properties of cationic microbubbles. In flow chamber studies, surface conjugation of cDNA did not affect attachment of targeted microbubbles at microvascular shear stresses (0.6 and 1.5 dyne/cm2). Attachment in vivo was also not affected by cDNA according to intravital microscopy observations of venular adhesion of ICAM-1-targeted microbubbles and by ultrasound molecular imaging of P-selectin-targeted microbubbles in the post-ischemic hindlimb in mice. Transfection at the site of high acoustic pressures (1.0 and 1.8 MPa) was similar for control and P-selectin-targeted microbubbles but was associated with vascular rupture and hemorrhage. At 0.6 MPa, there were no adverse bioeffects, and transfection was 5-fold greater with P-selectin-targeted microbubbles. Conclusions: We conclude that ultrasound-mediated transfection at safe acoustic pressures can be markedly augmented by endothelial juxtaposition.

Original languageEnglish (US)
Pages (from-to)1253-1262
Number of pages10
JournalJACC: Cardiovascular Imaging
Volume5
Issue number12
DOIs
StatePublished - Dec 2012

Fingerprint

Microbubbles
Transfection
Blood Vessels
P-Selectin
Genes
Complementary DNA
Acoustics
Intercellular Adhesion Molecule-1
Hindlimb
Ultrasonography
Pressure
Abdominal Muscles
Molecular Imaging
Optical Imaging
Luciferases
Genetic Therapy
Rupture

Keywords

  • contrast ultrasound
  • gene delivery
  • microbubbles

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Ultrasound-mediated vascular gene transfection by cavitation of endothelial-targeted cationic microbubbles. / Xie, Aris; Belcik, Todd; Yue, Qi; Morgan, Terry; Champaneri, Shivam A.; Taylor, Sarah; Davidson, Brian P.; Zhao, Yan; Klibanov, Alexander L.; Kuliszewski, Michael A.; Leong-Poi, Howard; Ammi, Azzdine; Lindner, Jonathan.

In: JACC: Cardiovascular Imaging, Vol. 5, No. 12, 12.2012, p. 1253-1262.

Research output: Contribution to journalArticle

Xie, A, Belcik, T, Yue, Q, Morgan, T, Champaneri, SA, Taylor, S, Davidson, BP, Zhao, Y, Klibanov, AL, Kuliszewski, MA, Leong-Poi, H, Ammi, A & Lindner, J 2012, 'Ultrasound-mediated vascular gene transfection by cavitation of endothelial-targeted cationic microbubbles', JACC: Cardiovascular Imaging, vol. 5, no. 12, pp. 1253-1262. https://doi.org/10.1016/j.jcmg.2012.05.017
Xie, Aris ; Belcik, Todd ; Yue, Qi ; Morgan, Terry ; Champaneri, Shivam A. ; Taylor, Sarah ; Davidson, Brian P. ; Zhao, Yan ; Klibanov, Alexander L. ; Kuliszewski, Michael A. ; Leong-Poi, Howard ; Ammi, Azzdine ; Lindner, Jonathan. / Ultrasound-mediated vascular gene transfection by cavitation of endothelial-targeted cationic microbubbles. In: JACC: Cardiovascular Imaging. 2012 ; Vol. 5, No. 12. pp. 1253-1262.
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AU - Taylor, Sarah

AU - Davidson, Brian P.

AU - Zhao, Yan

AU - Klibanov, Alexander L.

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AU - Leong-Poi, Howard

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AU - Lindner, Jonathan

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N2 - Objectives: Ultrasound-mediated gene delivery can be amplified by acoustic disruption of microbubble carriers that undergo cavitation. We hypothesized that endothelial targeting of microbubbles bearing cDNA is feasible and, through optimizing proximity to the vessel wall, increases the efficacy of gene transfection. Background: Contrast ultrasound-mediated gene delivery is a promising approach for site-specific gene therapy, although there are concerns with the reproducibility of this technique and the safety when using high-power ultrasound. Methods: Cationic lipid-shelled decafluorobutane microbubbles bearing a targeting moiety were prepared and compared with nontargeted microbubbles. Microbubble targeting efficiency to endothelial adhesion molecules (P-selectin or intercellular adhesion molecule [ICAM]-1) was tested using in vitro flow chamber studies, intravital microscopy of tumor necrosis factor-alpha (TNF-A)-stimulated murine cremaster muscle, and targeted contrast ultrasound imaging of P-selectin in a model of murine limb ischemia. Ultrasound-mediated transfection of luciferase reporter plasmid charge coupled to microbubbles in the post-ischemic hindlimb muscle was assessed by in vivo optical imaging. Results: Charge coupling of cDNA to the microbubble surface was not influenced by the presence of targeting ligand, and did not alter the cavitation properties of cationic microbubbles. In flow chamber studies, surface conjugation of cDNA did not affect attachment of targeted microbubbles at microvascular shear stresses (0.6 and 1.5 dyne/cm2). Attachment in vivo was also not affected by cDNA according to intravital microscopy observations of venular adhesion of ICAM-1-targeted microbubbles and by ultrasound molecular imaging of P-selectin-targeted microbubbles in the post-ischemic hindlimb in mice. Transfection at the site of high acoustic pressures (1.0 and 1.8 MPa) was similar for control and P-selectin-targeted microbubbles but was associated with vascular rupture and hemorrhage. At 0.6 MPa, there were no adverse bioeffects, and transfection was 5-fold greater with P-selectin-targeted microbubbles. Conclusions: We conclude that ultrasound-mediated transfection at safe acoustic pressures can be markedly augmented by endothelial juxtaposition.

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