Ultrasonic wave propagation assessment of native cartilage explants and hydrogel scaffolds for tissue engineering

Sean Kohles, Shelley S. Mason, Anya P. Adams, Robert J. Berg, Jessica Blank, Fay Gibson, Johnathan Righetti, Iesha S. Washington, Asit K. Saha

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Non-destructive techniques characterising the mechanical properties of cells, tissues, and biomaterials provide baseline metrics for tissue engineering design. Ultrasonic wave propagation and attenuation has previously demonstrated the dynamics of extracellular matrix synthesis in chondrocyte-seeded hydrogel constructs. In this paper, we describe an ultrasonic method to analyse two of the construct elements used to engineer articular cartilage in real-time, native cartilage explants and an agarose biomaterial. Results indicated a similarity in wave propagation velocity ranges for both longitudinal (1500-1745 m/s) and transverse (350-950 m/s) waveforms. Future work will apply an acoustoelastic analysis to distinguish between the fluid and solid properties including the cell and matrix biokinetics as a validation of previous mathematical models.

Original languageEnglish (US)
Pages (from-to)296-307
Number of pages12
JournalInternational Journal of Biomedical Engineering and Technology
Volume10
Issue number3
DOIs
StatePublished - 2012
Externally publishedYes

Fingerprint

Ultrasonic propagation
Cartilage
Scaffolds (biology)
Tissue engineering
Biomaterials
Hydrogels
Wave propagation
Ultrasonics
Tissue
Mathematical models
Engineers
Mechanical properties
Fluids

Keywords

  • Acoustoelasticity
  • Bioengineering
  • Biomedical engineering
  • Cartilage biokinetics
  • Cartilage engineering
  • Hydrogel biomaterials
  • Transmission wave elasticity
  • Ultrasonic elasticity

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Ultrasonic wave propagation assessment of native cartilage explants and hydrogel scaffolds for tissue engineering. / Kohles, Sean; Mason, Shelley S.; Adams, Anya P.; Berg, Robert J.; Blank, Jessica; Gibson, Fay; Righetti, Johnathan; Washington, Iesha S.; Saha, Asit K.

In: International Journal of Biomedical Engineering and Technology, Vol. 10, No. 3, 2012, p. 296-307.

Research output: Contribution to journalArticle

Kohles, S, Mason, SS, Adams, AP, Berg, RJ, Blank, J, Gibson, F, Righetti, J, Washington, IS & Saha, AK 2012, 'Ultrasonic wave propagation assessment of native cartilage explants and hydrogel scaffolds for tissue engineering', International Journal of Biomedical Engineering and Technology, vol. 10, no. 3, pp. 296-307. https://doi.org/10.1504/IJBET.2012.050263
Kohles, Sean ; Mason, Shelley S. ; Adams, Anya P. ; Berg, Robert J. ; Blank, Jessica ; Gibson, Fay ; Righetti, Johnathan ; Washington, Iesha S. ; Saha, Asit K. / Ultrasonic wave propagation assessment of native cartilage explants and hydrogel scaffolds for tissue engineering. In: International Journal of Biomedical Engineering and Technology. 2012 ; Vol. 10, No. 3. pp. 296-307.
@article{ebb516e21ace4d349eae474ea3fbc6fe,
title = "Ultrasonic wave propagation assessment of native cartilage explants and hydrogel scaffolds for tissue engineering",
abstract = "Non-destructive techniques characterising the mechanical properties of cells, tissues, and biomaterials provide baseline metrics for tissue engineering design. Ultrasonic wave propagation and attenuation has previously demonstrated the dynamics of extracellular matrix synthesis in chondrocyte-seeded hydrogel constructs. In this paper, we describe an ultrasonic method to analyse two of the construct elements used to engineer articular cartilage in real-time, native cartilage explants and an agarose biomaterial. Results indicated a similarity in wave propagation velocity ranges for both longitudinal (1500-1745 m/s) and transverse (350-950 m/s) waveforms. Future work will apply an acoustoelastic analysis to distinguish between the fluid and solid properties including the cell and matrix biokinetics as a validation of previous mathematical models.",
keywords = "Acoustoelasticity, Bioengineering, Biomedical engineering, Cartilage biokinetics, Cartilage engineering, Hydrogel biomaterials, Transmission wave elasticity, Ultrasonic elasticity",
author = "Sean Kohles and Mason, {Shelley S.} and Adams, {Anya P.} and Berg, {Robert J.} and Jessica Blank and Fay Gibson and Johnathan Righetti and Washington, {Iesha S.} and Saha, {Asit K.}",
year = "2012",
doi = "10.1504/IJBET.2012.050263",
language = "English (US)",
volume = "10",
pages = "296--307",
journal = "International Journal of Biomedical Engineering and Technology",
issn = "1752-6418",
publisher = "Inderscience Enterprises Ltd",
number = "3",

}

TY - JOUR

T1 - Ultrasonic wave propagation assessment of native cartilage explants and hydrogel scaffolds for tissue engineering

AU - Kohles, Sean

AU - Mason, Shelley S.

AU - Adams, Anya P.

AU - Berg, Robert J.

AU - Blank, Jessica

AU - Gibson, Fay

AU - Righetti, Johnathan

AU - Washington, Iesha S.

AU - Saha, Asit K.

PY - 2012

Y1 - 2012

N2 - Non-destructive techniques characterising the mechanical properties of cells, tissues, and biomaterials provide baseline metrics for tissue engineering design. Ultrasonic wave propagation and attenuation has previously demonstrated the dynamics of extracellular matrix synthesis in chondrocyte-seeded hydrogel constructs. In this paper, we describe an ultrasonic method to analyse two of the construct elements used to engineer articular cartilage in real-time, native cartilage explants and an agarose biomaterial. Results indicated a similarity in wave propagation velocity ranges for both longitudinal (1500-1745 m/s) and transverse (350-950 m/s) waveforms. Future work will apply an acoustoelastic analysis to distinguish between the fluid and solid properties including the cell and matrix biokinetics as a validation of previous mathematical models.

AB - Non-destructive techniques characterising the mechanical properties of cells, tissues, and biomaterials provide baseline metrics for tissue engineering design. Ultrasonic wave propagation and attenuation has previously demonstrated the dynamics of extracellular matrix synthesis in chondrocyte-seeded hydrogel constructs. In this paper, we describe an ultrasonic method to analyse two of the construct elements used to engineer articular cartilage in real-time, native cartilage explants and an agarose biomaterial. Results indicated a similarity in wave propagation velocity ranges for both longitudinal (1500-1745 m/s) and transverse (350-950 m/s) waveforms. Future work will apply an acoustoelastic analysis to distinguish between the fluid and solid properties including the cell and matrix biokinetics as a validation of previous mathematical models.

KW - Acoustoelasticity

KW - Bioengineering

KW - Biomedical engineering

KW - Cartilage biokinetics

KW - Cartilage engineering

KW - Hydrogel biomaterials

KW - Transmission wave elasticity

KW - Ultrasonic elasticity

UR - http://www.scopus.com/inward/record.url?scp=84874168091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874168091&partnerID=8YFLogxK

U2 - 10.1504/IJBET.2012.050263

DO - 10.1504/IJBET.2012.050263

M3 - Article

AN - SCOPUS:84874168091

VL - 10

SP - 296

EP - 307

JO - International Journal of Biomedical Engineering and Technology

JF - International Journal of Biomedical Engineering and Technology

SN - 1752-6418

IS - 3

ER -