Ultradeep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in simian immunodeficiency virus-infected macaques

Benjamin N. Bimber, Benjamin J. Burwitz, Shelby O'Connor, Ann Detmer, Emma Gostick, Simon M. Lank, David A. Price, Austin Hughes, David O'Connor

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8+ T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.

Original languageEnglish (US)
Pages (from-to)8247-8253
Number of pages7
JournalJournal of virology
Volume83
Issue number16
DOIs
StatePublished - Aug 2009

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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