Ultradeep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in simian immunodeficiency virus-infected macaques

Benjamin N. Bimber, Benjamin Burwitz, Shelby O'Connor, Ann Detmer, Emma Gostick, Simon M. Lank, David A. Price, Austin Hughes, David O'Connor

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8+ T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.

Original languageEnglish (US)
Pages (from-to)8247-8253
Number of pages7
JournalJournal of Virology
Volume83
Issue number16
DOIs
StatePublished - Aug 2009
Externally publishedYes

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Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca
T-lymphocytes
T-Lymphocytes
HIV
immunosuppression
Human immunodeficiency virus
Virus Diseases
viruses
infection

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Ultradeep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in simian immunodeficiency virus-infected macaques. / Bimber, Benjamin N.; Burwitz, Benjamin; O'Connor, Shelby; Detmer, Ann; Gostick, Emma; Lank, Simon M.; Price, David A.; Hughes, Austin; O'Connor, David.

In: Journal of Virology, Vol. 83, No. 16, 08.2009, p. 8247-8253.

Research output: Contribution to journalArticle

Bimber, BN, Burwitz, B, O'Connor, S, Detmer, A, Gostick, E, Lank, SM, Price, DA, Hughes, A & O'Connor, D 2009, 'Ultradeep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in simian immunodeficiency virus-infected macaques', Journal of Virology, vol. 83, no. 16, pp. 8247-8253. https://doi.org/10.1128/JVI.00897-09
Bimber, Benjamin N. ; Burwitz, Benjamin ; O'Connor, Shelby ; Detmer, Ann ; Gostick, Emma ; Lank, Simon M. ; Price, David A. ; Hughes, Austin ; O'Connor, David. / Ultradeep pyrosequencing detects complex patterns of CD8+ T-lymphocyte escape in simian immunodeficiency virus-infected macaques. In: Journal of Virology. 2009 ; Vol. 83, No. 16. pp. 8247-8253.
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