TY - JOUR
T1 - Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing
AU - Adey, Andrew
AU - Shendure, Jay
PY - 2012/6
Y1 - 2012/6
N2 - We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6x high-quality coverage of each strand.
AB - We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6x high-quality coverage of each strand.
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U2 - 10.1101/gr.136242.111
DO - 10.1101/gr.136242.111
M3 - Article
C2 - 22466172
AN - SCOPUS:84861904633
SN - 1088-9051
VL - 22
SP - 1139
EP - 1143
JO - PCR Methods and Applications
JF - PCR Methods and Applications
IS - 6
ER -