Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing

Andrew Adey, Jay Shendure

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6x high-quality coverage of each strand.

Original languageEnglish (US)
Pages (from-to)1139-1143
Number of pages5
JournalGenome Research
Volume22
Issue number6
DOIs
StatePublished - Jun 2012
Externally publishedYes

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Genome
Transposases
DNA
Firearms
Libraries
Cell Line
hydrogen sulfite
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing. / Adey, Andrew; Shendure, Jay.

In: Genome Research, Vol. 22, No. 6, 06.2012, p. 1139-1143.

Research output: Contribution to journalArticle

Adey, Andrew ; Shendure, Jay. / Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing. In: Genome Research. 2012 ; Vol. 22, No. 6. pp. 1139-1143.
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