Ultra-high throughput isolation of circulating tumor cells with microfluidic vortex technology

J. Che, M. Dhar, V. Yu, D. E. Go, E. Pao, M. Matsumoto, E. B. Garon, J. Goldman, R. P. Kulkarni, E. Sollier, D. Di Carlo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Here we describe improvements for the rapid, size-based capture of circulating tumor cells (CTCs) from blood. We have previously presented the Vortex Chip [1], an inertial microfluidic device which isolates CTCs at high throughput (400 μL/min of whole blood), purity (>80%), and viability (∼80%), but was limited by ∼20% efficiency. Here, we demonstrate improved CTC capture efficiency (up to 40%) and speed of processing (up to 8 mL/min) with the High Throughput Vortex Chip (Vortex HT). We show in cancer cell lines and clinical samples that the Vortex HT purifies larger numbers of CTCs.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages618-620
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Cancer cell trapping
  • Cancer prognosis
  • Inertial microfluidics
  • Medical devices
  • Rare cell enrichment
  • Size-based purification
  • Vortex

ASJC Scopus subject areas

  • Control and Systems Engineering

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