@article{c4cc2943004d43c5add06e269aff9e2f,
title = "Ultra-fast conductive media for RNA electrophoretic mobility shift assays",
abstract = "The use of RNA electrophoretic mobility shift assays (REMSAs) for analysis of RNA-protein interactions have been limited to lengthy assay time and qualitative assessment. To vastly improve assay efficiency, feasibility and quality of data procured from REMSAs, we combine here some of the best-known labeling and electrophoretic techniques. Nucleic acid fragments are end-labeled with fluorescent tags, as opposed to the radioactive or biotin tags. The fluorescent probes may be detected directly from the electrophoresis gel, eliminating the need for cumbersome membrane transfer and immunoblotting. Modifying the REMSA protocol to include low-molarity, lithium borate conductive media and near-infrared-labeled probes allows for a reduction assay time, quantitative comparison between experimental conditions and crisp band resolution (i.e., optimized results).",
keywords = "*Author for, Conductive media, ELAVL1, EMSA, HuR, IR-oligonucleotides, LB, RBP, REMSA",
author = "Brown, {Samantha Z.} and Agostini, {Lebaron C.} and Thomsett, {Henry L.} and Brody, {Jonathan R.}",
note = "Funding Information: This work was supported by National Institutes of Health grant number 1R01CA212600-01, and the Sarah Parvin Foundation. JR Brody is the co-owner of Faster Better Media LLC and as such has received revenue on sales of products describedinthisarticle.Thiscreatesasignificant financial conflict of interest, which is being managed by The Johns Hopkins UniversityandJeffersonUniversityinaccordance with its conflict of interest policies.Theauthorshavenootherrelevant affiliationsorfinancialinvolvementwithany organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Funding Information: This work was supported by National Institutes of Health grant number 1R01CA212600-01, and the Sarah Parvin Foundation. JR Brody is the co-owner of Faster Better Media LLC and as such has received revenue on sales of products described in this article. This creates a significant ?nancial con ?ict of interest, which is•• being managed by The Johns Hopkins University and Jefferson University in accordance with its conflict of interest policies. The authors have no other relevant a ?liations or ?nancial involvement with any Publisher Copyright: {\textcopyright} 2019 Jonathan R. Brody Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
doi = "10.2144/btn-2019-0111",
language = "English (US)",
volume = "68",
pages = "101--105",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "2",
}