Ubiquitous sialometabolism present among oral fusobacteria

Saori Yoneda, Brandon Loeser, Joseph Feng, John Dmytryk, Fengxia Qi, Justin Merritt

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fusobacterium nucleatum is a ubiquitous member of the human oral flora and is associated with the development of periodontitis and a variety of other types of polymicrobial infections of the mucosa. In the oral cavity, this species is one of the few that is prevalent in both healthy and diseased subgingival plaque. Using microarray analysis, we examined the transcriptional response of F. nucleatum subspecies nucleatum to whole blood in order to identify some of the genetic responses that might occur during the transition from health to disease. From these studies, we identified a sialic acid catabolism operon that was induced by the presence of blood. We subsequently confirmed that this operon was inducible by the presence of synthetic sialic acid, but we found no evidence suggesting sialic acid was used as a major carbon source. However, this organism was found to possess a de novo synthesized surface sialylation ability that is widely conserved among the various F. nucleatum subspecies as well as in F. periodonticum. We provide evidence that fusobacterial sialylation does occur in the oral cavity irrespective of health status. Interestingly, only a minority of fusobacterial cells exhibit surface sialylation within dental plaque, whereas most cells are uniformly sialylated when grown in pure culture. The implications of these results are discussed.

Original languageEnglish (US)
Article numbere99263
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 4 2014
Externally publishedYes

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Fusobacteria
Fusobacterium nucleatum
sialic acids
N-Acetylneuraminic Acid
mouth
operon
Operon
Mouth
Blood
Health Transition
Health
Dental Plaque
Periodontitis
blood
Microarray Analysis
Microarrays
Coinfection
health status
Health Status
mucosa

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ubiquitous sialometabolism present among oral fusobacteria. / Yoneda, Saori; Loeser, Brandon; Feng, Joseph; Dmytryk, John; Qi, Fengxia; Merritt, Justin.

In: PLoS One, Vol. 9, No. 6, e99263, 04.06.2014.

Research output: Contribution to journalArticle

Yoneda, S, Loeser, B, Feng, J, Dmytryk, J, Qi, F & Merritt, J 2014, 'Ubiquitous sialometabolism present among oral fusobacteria', PLoS One, vol. 9, no. 6, e99263. https://doi.org/10.1371/journal.pone.0099263
Yoneda, Saori ; Loeser, Brandon ; Feng, Joseph ; Dmytryk, John ; Qi, Fengxia ; Merritt, Justin. / Ubiquitous sialometabolism present among oral fusobacteria. In: PLoS One. 2014 ; Vol. 9, No. 6.
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