Tyrosine phosphorylation of rasGAP and associated proteins in chronic myelogenous leukemia cell lines

Brian Druker, Keiko Okuda, Ursula Matulonis, Ravi Salgia, Thomas Roberts, James D. Griffin

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The Philadelphia chromosome (Ph1), detected in virtually all cases of chronic myelogenous leukemia, is formed by a reciprocal translocation between chromosomes 9 and 22 that fuses BCR encoded sequences upstream of exon 2 of c-ABL. This oncogene produces a fusion protein (p210BCR/ABL) in which the ABL tyrosine kinase activity is elevated. This elevated kinase activity is essential for transformation, but the mechanisms involved are unknown. We report here that p21ras GTPase activating protein (rasGAP) or rasGAP-associated proteins p190 and p62 are phosphorated on tyrosine in Ph1 (+) cell lines. Further, rasGAP coimmunoprecipitates with p210BCR/ABL in these cell lines. These results suggest that rasGAP or associated proteins are potential substrates for p210BCR/ABL kinase and thus directly link p210BCR/ABL With a signal transduction pathway known to be activated by hematopoietic growth factors (p21ras).

Original languageEnglish (US)
Pages (from-to)2215-2220
Number of pages6
JournalBlood
Volume79
Issue number9
StatePublished - May 1 1992
Externally publishedYes

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Phosphorylation
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Tyrosine
Cells
Chromosomes
Cell Line
Phosphotransferases
GTPase-Activating Proteins
Chromosomes, Human, Pair 22
Philadelphia Chromosome
Signal transduction
Chromosomes, Human, Pair 9
Proteins
Electric fuses
Oncogenes
Protein-Tyrosine Kinases
Exons
Signal Transduction
Intercellular Signaling Peptides and Proteins
Fusion reactions

ASJC Scopus subject areas

  • Hematology

Cite this

Druker, B., Okuda, K., Matulonis, U., Salgia, R., Roberts, T., & Griffin, J. D. (1992). Tyrosine phosphorylation of rasGAP and associated proteins in chronic myelogenous leukemia cell lines. Blood, 79(9), 2215-2220.

Tyrosine phosphorylation of rasGAP and associated proteins in chronic myelogenous leukemia cell lines. / Druker, Brian; Okuda, Keiko; Matulonis, Ursula; Salgia, Ravi; Roberts, Thomas; Griffin, James D.

In: Blood, Vol. 79, No. 9, 01.05.1992, p. 2215-2220.

Research output: Contribution to journalArticle

Druker, B, Okuda, K, Matulonis, U, Salgia, R, Roberts, T & Griffin, JD 1992, 'Tyrosine phosphorylation of rasGAP and associated proteins in chronic myelogenous leukemia cell lines', Blood, vol. 79, no. 9, pp. 2215-2220.
Druker B, Okuda K, Matulonis U, Salgia R, Roberts T, Griffin JD. Tyrosine phosphorylation of rasGAP and associated proteins in chronic myelogenous leukemia cell lines. Blood. 1992 May 1;79(9):2215-2220.
Druker, Brian ; Okuda, Keiko ; Matulonis, Ursula ; Salgia, Ravi ; Roberts, Thomas ; Griffin, James D. / Tyrosine phosphorylation of rasGAP and associated proteins in chronic myelogenous leukemia cell lines. In: Blood. 1992 ; Vol. 79, No. 9. pp. 2215-2220.
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