Tyrosine phosphorylation of α tubulin in human T lymphocytes

Steven C. Ley, Winston Verbi, Darryl J C Pappin, Brian Druker, Adelina A. Davies, Michael J. Crumpton

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

N-terminal sequencing of the 55- and 50-kDa polypeptides affinity purified on a phosphotyrosine monoclonal antibody column from activated Jurkat T cells identified α and β tubulin. Two-dimensional gel analysis indicated that α tubulin was directly phosphorylated on tyrosine. β Tubulin was not detectably tyrosine phosphorylated but was precipitated by anti-phosphotyrosine (PTyr) antibody by virtue of its association with the α subunit as a heterodimer. Phosphotyrosyl α tubulin was not incorporated into intact microtubules and was all in the unpolymerized soluble fraction. These results suggest that tyrosine phosphorylation of α tubulin may inhibit the ability of this subunit to polymerize into microtubules. Stimulation of Jurkat T cells via T cell receptor increased the amount of tubulin precipitated by the anti-PTyr antibody. These data raise the possibility that the polymerization of tubulin heterodimers may be regulated by phosphorylation on tyrosine during T cell activation.

Original languageEnglish (US)
Pages (from-to)99-106
Number of pages8
JournalEuropean Journal of Immunology
Volume24
Issue number1
DOIs
StatePublished - Jan 1994
Externally publishedYes

Fingerprint

Tubulin
Phosphorylation
T-Lymphocytes
Phosphotyrosine
Tyrosine
Jurkat Cells
Microtubules
Anti-Idiotypic Antibodies
T-Cell Antigen Receptor
Polymerization
tyrosine-tubulin
Gels
Monoclonal Antibodies
Peptides

Keywords

  • α Tubulin
  • T lymphocytes
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Immunology

Cite this

Ley, S. C., Verbi, W., Pappin, D. J. C., Druker, B., Davies, A. A., & Crumpton, M. J. (1994). Tyrosine phosphorylation of α tubulin in human T lymphocytes. European Journal of Immunology, 24(1), 99-106. https://doi.org/10.1002/eji.1830240116

Tyrosine phosphorylation of α tubulin in human T lymphocytes. / Ley, Steven C.; Verbi, Winston; Pappin, Darryl J C; Druker, Brian; Davies, Adelina A.; Crumpton, Michael J.

In: European Journal of Immunology, Vol. 24, No. 1, 01.1994, p. 99-106.

Research output: Contribution to journalArticle

Ley, SC, Verbi, W, Pappin, DJC, Druker, B, Davies, AA & Crumpton, MJ 1994, 'Tyrosine phosphorylation of α tubulin in human T lymphocytes', European Journal of Immunology, vol. 24, no. 1, pp. 99-106. https://doi.org/10.1002/eji.1830240116
Ley, Steven C. ; Verbi, Winston ; Pappin, Darryl J C ; Druker, Brian ; Davies, Adelina A. ; Crumpton, Michael J. / Tyrosine phosphorylation of α tubulin in human T lymphocytes. In: European Journal of Immunology. 1994 ; Vol. 24, No. 1. pp. 99-106.
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