Type VII collagen is required for ras-driven human epidermal tumorigenesis

Susana Ortiz-Urda, John Garcia, Cheryl L. Green, Lei Chen, Qun Lin, Dallas P. Veitch, Lynn Sakai, Hyangkyu Lee, M. Peter Marinkovich, Paul A. Khavari

Research output: Contribution to journalArticle

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Abstract

Type VII collagen defects cause recessive dystrophic epidermolysis bullosa (RDEB), a blistering skin disorder often accompanied by epidermal cancers. To study the role of collagen VII in these cancers, we examined Ras-driven tumorigenesis in RDEB keratinocytes. Cells devoid of collagen VII did not form tumors in mice, whereas those retaining a specific collagen VII fragment (the amino-terminal noncollagenous domain NC1) were tumorigenic. Forced NC1 expression restored tumorigenicity to collagen VII-null epidermis in a nori-cell-autonomous fashion. Fibronectin-like sequences within NC1 (FNC1) promoted tumor cell invasion in a laminin 5-dependent manner and were required for tumorigenesis. Tumor-stroma interactions mediated by collagen VII thus promote neoplasia, and retention of NC1 sequences in a subset of RDEB patients may contribute to their increased susceptibility to squamous cell carcinoma.

Original languageEnglish (US)
Pages (from-to)1773-1776
Number of pages4
JournalScience
Volume307
Issue number5716
DOIs
StatePublished - Mar 18 2005

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Collagen Type VII
Carcinogenesis
Epidermolysis Bullosa Dystrophica
Collagen
Neoplasms
Porphyra
Keratinocytes
Fibronectins
Epidermis
Squamous Cell Carcinoma
Skin

ASJC Scopus subject areas

  • General

Cite this

Ortiz-Urda, S., Garcia, J., Green, C. L., Chen, L., Lin, Q., Veitch, D. P., ... Khavari, P. A. (2005). Type VII collagen is required for ras-driven human epidermal tumorigenesis. Science, 307(5716), 1773-1776. https://doi.org/10.1126/science.1106209

Type VII collagen is required for ras-driven human epidermal tumorigenesis. / Ortiz-Urda, Susana; Garcia, John; Green, Cheryl L.; Chen, Lei; Lin, Qun; Veitch, Dallas P.; Sakai, Lynn; Lee, Hyangkyu; Marinkovich, M. Peter; Khavari, Paul A.

In: Science, Vol. 307, No. 5716, 18.03.2005, p. 1773-1776.

Research output: Contribution to journalArticle

Ortiz-Urda, S, Garcia, J, Green, CL, Chen, L, Lin, Q, Veitch, DP, Sakai, L, Lee, H, Marinkovich, MP & Khavari, PA 2005, 'Type VII collagen is required for ras-driven human epidermal tumorigenesis', Science, vol. 307, no. 5716, pp. 1773-1776. https://doi.org/10.1126/science.1106209
Ortiz-Urda S, Garcia J, Green CL, Chen L, Lin Q, Veitch DP et al. Type VII collagen is required for ras-driven human epidermal tumorigenesis. Science. 2005 Mar 18;307(5716):1773-1776. https://doi.org/10.1126/science.1106209
Ortiz-Urda, Susana ; Garcia, John ; Green, Cheryl L. ; Chen, Lei ; Lin, Qun ; Veitch, Dallas P. ; Sakai, Lynn ; Lee, Hyangkyu ; Marinkovich, M. Peter ; Khavari, Paul A. / Type VII collagen is required for ras-driven human epidermal tumorigenesis. In: Science. 2005 ; Vol. 307, No. 5716. pp. 1773-1776.
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