Type VII collagen is a major structural component of anchoring fibrils

Lynn Sakai, D. R. Keene, N. P. Morris, R. E. Burgeson

Research output: Contribution to journalArticle

373 Citations (Scopus)

Abstract

Anchoring fibrils are specialized fibrous structures found in the subbasal lamina underlying epithelia of several external tissues. Based upon their sensitivity to collagenase and the similarity in banding pattern to artificially created segment-long spacing crystallites (SLS) of collagens, several authors have suggested that anchoring fibrils are lateral aggregates of collagenous macromolecules. We recently reported the similarity in length and banding pattern of anchoring fibrils to type VII collagen SLS crystallites. We now report the construction and characterization of a murine monoclonal antibody specific for type VII collagen. The epitope identified by this antibody has been mapped to the carboxyl terminus of the major helical domain of this molecule. The presence of type VII collagen as detected by indirect immunofluorescence in a variety of tissues corresponds exactly with ultrastructural observations of anchoring fibrils. Ultrastructural immunolocalization of type VII collagen using a 5-nm colloidal gold-conjugated second antibody demonstrates metal deposition upon anchoring fibrils at both ends of these structures, as predicted by the location of the epitope on type VII collagen. Type VII collagen is synthesized by primary cultures of amniotic epithelial cells. It is also produced by KB cells (an epidermoid carcinoma cell line) and WISH (a transformed amniotic cell line).

Original languageEnglish (US)
Pages (from-to)1577-1586
Number of pages10
JournalJournal of Cell Biology
Volume103
Issue number4
DOIs
StatePublished - Jan 1 1986

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Collagen Type VII
Epitopes
KB Cells
Gold Colloid
Transformed Cell Line
Antibodies
Collagenases
Indirect Fluorescent Antibody Technique
Squamous Cell Carcinoma
Collagen
Epithelium
Epithelial Cells
Metals
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Cell Biology

Cite this

Type VII collagen is a major structural component of anchoring fibrils. / Sakai, Lynn; Keene, D. R.; Morris, N. P.; Burgeson, R. E.

In: Journal of Cell Biology, Vol. 103, No. 4, 01.01.1986, p. 1577-1586.

Research output: Contribution to journalArticle

Sakai, Lynn ; Keene, D. R. ; Morris, N. P. ; Burgeson, R. E. / Type VII collagen is a major structural component of anchoring fibrils. In: Journal of Cell Biology. 1986 ; Vol. 103, No. 4. pp. 1577-1586.
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