Type III collagen can be present on banded collagen fibrils regardless of fibril diameter

D. R. Keene, Lynn Sakai, H. P. Bachinger, R. E. Burgerson

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Monoclonal antibodies that recognize an epitope within the triple helix of type III collagen have been used to examine the distribution of that collagen type in human skin, cornea, amnion, aorta, and tendon. Ultrastructural examination of those tissues indicates antibody binding to collagen fibrils in skin, amnion, aorta, and tendon regardless of the diameter of the fibril. The antibody distribution is unchanged with donor age, site of biopsy, or region of tissue examined. In contrast, antibody applied to adult human cornea localizes to isolated fibrils, which appear randomly throughout the matrix. These studies indicate that type III collagen remains associated with collagen fibrils after removal of the amino and carboxyl propeptides, and suggests that fibrils of skin, tendon, and amnion (and presumably many other tissues that contain both types I and III collagens) are copolymers of at least types I and III collagens.

Original languageEnglish (US)
Pages (from-to)2393-2402
Number of pages10
JournalJournal of Cell Biology
Volume105
Issue number5
DOIs
StatePublished - Jan 1 1987

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Collagen Type III
Amnion
Collagen
Tendons
Collagen Type I
Cornea
Skin
Aorta
Antibodies
Fibril-Associated Collagens
Epitopes
Monoclonal Antibodies
Tissue Donors
Biopsy

ASJC Scopus subject areas

  • Cell Biology

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Type III collagen can be present on banded collagen fibrils regardless of fibril diameter. / Keene, D. R.; Sakai, Lynn; Bachinger, H. P.; Burgerson, R. E.

In: Journal of Cell Biology, Vol. 105, No. 5, 01.01.1987, p. 2393-2402.

Research output: Contribution to journalArticle

Keene, D. R. ; Sakai, Lynn ; Bachinger, H. P. ; Burgerson, R. E. / Type III collagen can be present on banded collagen fibrils regardless of fibril diameter. In: Journal of Cell Biology. 1987 ; Vol. 105, No. 5. pp. 2393-2402.
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