Type I adenylyl cyclase functions as a coincidence detector for control of cyclic AMP response element-mediated transcription: Synergistic regulation of transcription by Ca2+ and isoproterenol

S. Impey, G. Wayman, Z. Wu, D. R. Storm

Research output: Contribution to journalArticle

53 Scopus citations


Studies carried out with mammals and invertebrates suggest that Ca2+- sensitive adenylyl cyclases may be important for neuroplasticity. Long-term potentiation in the hippocampus requires increases in intracellular Ca2+ which are accompanied by elevated cyclic AMP (cAMP). Furthermore, activation of cAMP-dependent protein kinase is required for the late stage of long-term potentiation in the CA1 region of the hippocampus, which is also sensitive to inhibitors of transcription. Therefore, some forms of synaptic plasticity may require coordinate regulation of transcription by Ca2+ and cAMP. In this study, we demonstrate that the expression of type I adenylyl cyclase in HEK- 293 cells allows Ca2+ to stimulate reporter gene activity mediated through the cAMP response element. Furthermore, simultaneous activation by Ca2+ and isoproterenol caused synergistic stimulation of transcription in HEK-293 cells and cultured neurons. We propose that Ca2+ and neurotransmitter stimulation of type I adenylyl cyclase may play a role in synaptic plasticity by generating optimal cAMP signals for regulation of transcription.

Original languageEnglish (US)
Pages (from-to)8272-8281
Number of pages10
JournalMolecular and cellular biology
Issue number12
StatePublished - Jan 1 1994


ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this