TY - JOUR
T1 - Two ribeye genes in teleosts
T2 - The role of ribeye in ribbon formation and bipolar cell development
AU - Wan, Lei
AU - Almers, Wolfhard
AU - Chen, Wenbiao
PY - 2005/2/26
Y1 - 2005/2/26
N2 - Ribeye is the only known protein specific to synaptic ribbon, but its function is unclear. We show that the teleost fish, Fugu and zebrafish, have two ribeye genes, ribeye a and ribeye b. Whole-mount in situ hybridization revealed that ribeye a is expressed in tissues containing synaptic ribbons, including the pineal gland, inner ear, and retina. Ribeye b is absent in the pineal gland. In the retina, ribeye a is expressed in both photoreceptors and bipolar cells, whereas ribeye b is detected only in photoreceptors. To study the function of Ribeye a in retina, we depleted it by morpholino antisense oligos. Fish deficient in Ribeye a lack an optokinetic response and have shorter synaptic ribbons in photoreceptors and fewer synaptic ribbons in bipolar cells. Their bipolar cells still target Syntaxin-3 proteins to the inner plexiform layer and have abundant vsx1 mRNA. However, they lack large synaptic terminals and show increased apoptosis. Rod bipolar cells are fewer in number and/or deficient in PKCα. Recovery of Ribeye a levels rescues the optokinetic response, increases the number of PKCα-positive bipolar cells, and stops apoptosis. We conclude that Ribeye a is important for late steps in bipolar cell development.
AB - Ribeye is the only known protein specific to synaptic ribbon, but its function is unclear. We show that the teleost fish, Fugu and zebrafish, have two ribeye genes, ribeye a and ribeye b. Whole-mount in situ hybridization revealed that ribeye a is expressed in tissues containing synaptic ribbons, including the pineal gland, inner ear, and retina. Ribeye b is absent in the pineal gland. In the retina, ribeye a is expressed in both photoreceptors and bipolar cells, whereas ribeye b is detected only in photoreceptors. To study the function of Ribeye a in retina, we depleted it by morpholino antisense oligos. Fish deficient in Ribeye a lack an optokinetic response and have shorter synaptic ribbons in photoreceptors and fewer synaptic ribbons in bipolar cells. Their bipolar cells still target Syntaxin-3 proteins to the inner plexiform layer and have abundant vsx1 mRNA. However, they lack large synaptic terminals and show increased apoptosis. Rod bipolar cells are fewer in number and/or deficient in PKCα. Recovery of Ribeye a levels rescues the optokinetic response, increases the number of PKCα-positive bipolar cells, and stops apoptosis. We conclude that Ribeye a is important for late steps in bipolar cell development.
KW - Apoptosis
KW - Bipolar cells
KW - Morpholino antisense oligo
KW - Optokinetic response
KW - Retina
KW - Ribeye
KW - Synaptic ribbon
KW - Synaptogenesis
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UR - http://www.scopus.com/inward/citedby.url?scp=12844256493&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.4657-04.2005
DO - 10.1523/JNEUROSCI.4657-04.2005
M3 - Article
C2 - 15673675
AN - SCOPUS:12844256493
SN - 0270-6474
VL - 25
SP - 941
EP - 949
JO - Journal of Neuroscience
JF - Journal of Neuroscience
IS - 4
ER -