Two distinct sequence elements mediate retroviral gene expression in embryonal carcinoma cells

H. Weiher, E. Barklis, W. Ostertag, R. Jaenisch

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Moloney murine leukemia virus (M-MuLV) and M-MuLV-derived retroviral vectors are not expressed in early mouse embryos or in embryonal carcinoma cells. M-MuLV-derived mutants or M-MuLV-related variants which transduce the neomycin phosphotransferase gene can, however, induce drug resistance in embryonal carcinoma cells with high efficiency. In this study we investigated the sequences critical for retroviral gene expression in two different embryonal carcinoma cell lines, F9 and PCC4. We show that two synergistically acting sequence elements mediate expression in embryonal carcinoma cells. One of these is located within the U3 region of the viral long terminal repeat, and the second one is in the 5' untranslated region of the retrovirus. The latter element, characterized by a single point mutation, affects the level of stable RNA in infected cells, suggesting a regulatory mechanism similar to that of human immunodeficiency virus in human T cells.

Original languageEnglish (US)
Pages (from-to)2742-2746
Number of pages5
JournalJournal of virology
Volume61
Issue number9
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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