Two distinct mechanisms target membrane proteins to the axonal surface

Bernard Sampo, Stefanie Kaech-Petrie, Stefan Kunz, Gary Banker

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

We have investigated the trafficking of two endogenous axonal membrane proteins, VAMP2 and NgCAM, in order to elucidate the cellular events that underlie their polarization. We found that VAMP2 is delivered to the surface of both axons and dendrites, but preferentially endocytosed from the dendritic membrane. A mutation in the cytoplasmic domain of VAMP2 that inhibits endocytosis abolished its axonal polarization. In contrast, the targeting of NgCAM depends on sequences in its ectodomain, which mediate its sorting into carriers that preferentially deliver their cargo proteins to the axonal membrane. These observations show that neurons use two distinct mechanisms to polarize proteins to the axonal domain: selective retention in the case of VAMP2, selective delivery in the case of NgCAM.

Original languageEnglish (US)
Pages (from-to)611-624
Number of pages14
JournalNeuron
Volume37
Issue number4
DOIs
StatePublished - Feb 20 2003

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Vesicle-Associated Membrane Protein 2
Membrane Proteins
Endocytosis
Membranes
Dendrites
Axons
Proteins
Neurons
Mutation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Two distinct mechanisms target membrane proteins to the axonal surface. / Sampo, Bernard; Kaech-Petrie, Stefanie; Kunz, Stefan; Banker, Gary.

In: Neuron, Vol. 37, No. 4, 20.02.2003, p. 611-624.

Research output: Contribution to journalArticle

Sampo, Bernard ; Kaech-Petrie, Stefanie ; Kunz, Stefan ; Banker, Gary. / Two distinct mechanisms target membrane proteins to the axonal surface. In: Neuron. 2003 ; Vol. 37, No. 4. pp. 611-624.
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