Two-dimensional liquid chromatography study of the human whole saliva proteome

Phillip A. Wilmarth, Michael A. Riviere, D. Leif Rustvold, Jeffrey D. Lauten, Theresa E. Madden, Larry L. David

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

The human whole saliva proteome was investigated using two-dimensional liquid chromatography (2-DLC). The 2-DLC study was able to identify, with high confidence, 102 proteins including most known salivary proteins (35), and a large number of common serum proteins (67). Peptides from proline-rich proteins, abundant in saliva, had unusual cleavage sites and were frequently only partially tryptic. Three proteins not previously observed in human saliva were also detected. Significantly greater numbers of identified proteins, including high molecular weight, low molecular weight, and proline-rich proteins, were found with 2-DLC compared to previously reported two-dimensional gel electrophoresis studies.

Original languageEnglish (US)
Pages (from-to)1017-1023
Number of pages7
JournalJournal of Proteome Research
Volume3
Issue number5
DOIs
StatePublished - Sep 1 2004

Keywords

  • Human saliva
  • Mass spectrometry
  • Two-dimensional liquid chromatography

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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