Two-color, one-photon uncaging of glutamate and GABA

Stefan Passlick, Paul F. Kramer, Matthew T. Richers, John Williams, Graham C.R. Ellis-Davies

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Neuronal cells receive a variety of excitatory and inhibitory signals which they process to generate an output signal. In order to study the interaction between excitatory and inhibitory receptors with exogenously applied transmitters in the same preparation, two caging chromophores attached to glutamate and GABA were developed that were selectively photolyzed by different wavelengths of light. This technique has the advantage that the biologically inactive caged compound can be applied at equilibrium prior to the near instantaneous release of the transmitters. This method therefore mimics the kinetics of endogenously released transmitters that is otherwise not possible in brain slice preparations. Repeated photolysis with either of the two wavelengths resulted in GABA- or glutamate-induced activation of both ionotropic and metabotropic receptors to evoke reproducible currents. With these compounds, the interaction between inhibitory and excitatory receptors was examined using whole field photolysis.

Original languageEnglish (US)
Article numbere0187732
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017

Fingerprint

Photolysis
Photons
glutamates
gamma-Aminobutyric Acid
Glutamic Acid
Transmitters
Color
photolysis
receptors
wavelengths
color
Wavelength
Chromophores
Light
Brain
neurons
Chemical activation
brain
kinetics
Kinetics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Passlick, S., Kramer, P. F., Richers, M. T., Williams, J., & Ellis-Davies, G. C. R. (2017). Two-color, one-photon uncaging of glutamate and GABA. PLoS One, 12(11), [e0187732]. https://doi.org/10.1371/journal.pone.0187732

Two-color, one-photon uncaging of glutamate and GABA. / Passlick, Stefan; Kramer, Paul F.; Richers, Matthew T.; Williams, John; Ellis-Davies, Graham C.R.

In: PLoS One, Vol. 12, No. 11, e0187732, 01.11.2017.

Research output: Contribution to journalArticle

Passlick, S, Kramer, PF, Richers, MT, Williams, J & Ellis-Davies, GCR 2017, 'Two-color, one-photon uncaging of glutamate and GABA', PLoS One, vol. 12, no. 11, e0187732. https://doi.org/10.1371/journal.pone.0187732
Passlick S, Kramer PF, Richers MT, Williams J, Ellis-Davies GCR. Two-color, one-photon uncaging of glutamate and GABA. PLoS One. 2017 Nov 1;12(11). e0187732. https://doi.org/10.1371/journal.pone.0187732
Passlick, Stefan ; Kramer, Paul F. ; Richers, Matthew T. ; Williams, John ; Ellis-Davies, Graham C.R. / Two-color, one-photon uncaging of glutamate and GABA. In: PLoS One. 2017 ; Vol. 12, No. 11.
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