Twist-off purification of hair bundles

Jung Bum Shin, James Pagana, Peter Barr-Gillespie

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Purification of hair bundles from inner-ear organs allows biochemical analysis of bundle constituents, including proteins and lipids. We describe here the "twist-off" method of bundle isolation, where dissected inner-ear organs are embedded in agarose, then subjected to a mechanical disruption that shears off bundles and leaves them in agarose blocks. With care in the dissection and in clean-up of the isolated bundles, contamination from cell bodies can be kept to a minimum. Isolated bundles can be analyzed by a variety of techniques, including immunocytochemistry, SDS-PAGE, immunoblotting, and mass spectrometry.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages241-255
Number of pages15
Volume493
ISBN (Print)9781934115626
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume493
ISSN (Print)10643745

Fingerprint

Inner Ear
Sepharose
Immunoblotting
Dissection
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Immunohistochemistry
Lipids
Proteins
Cell Body

Keywords

  • Agarose
  • Auditory
  • Hair bundles
  • Mass spectrometry
  • Mechanotransduction
  • Vestibular

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Shin, J. B., Pagana, J., & Barr-Gillespie, P. (2009). Twist-off purification of hair bundles. In Methods in Molecular Biology (Vol. 493, pp. 241-255). (Methods in Molecular Biology; Vol. 493). Humana Press. https://doi.org/10.1007/978-1-59745-523-7_14

Twist-off purification of hair bundles. / Shin, Jung Bum; Pagana, James; Barr-Gillespie, Peter.

Methods in Molecular Biology. Vol. 493 Humana Press, 2009. p. 241-255 (Methods in Molecular Biology; Vol. 493).

Research output: Chapter in Book/Report/Conference proceedingChapter

Shin, JB, Pagana, J & Barr-Gillespie, P 2009, Twist-off purification of hair bundles. in Methods in Molecular Biology. vol. 493, Methods in Molecular Biology, vol. 493, Humana Press, pp. 241-255. https://doi.org/10.1007/978-1-59745-523-7_14
Shin JB, Pagana J, Barr-Gillespie P. Twist-off purification of hair bundles. In Methods in Molecular Biology. Vol. 493. Humana Press. 2009. p. 241-255. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-523-7_14
Shin, Jung Bum ; Pagana, James ; Barr-Gillespie, Peter. / Twist-off purification of hair bundles. Methods in Molecular Biology. Vol. 493 Humana Press, 2009. pp. 241-255 (Methods in Molecular Biology).
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