@inbook{01375663bdc24bc999439f29bc82701b,
title = "Twist-off purification of hair bundles",
abstract = "Purification of hair bundles from inner-ear organs allows biochemical analysis of bundle constituents, including proteins and lipids. We describe here the {"}twist-off{"} method of bundle isolation, where dissected inner-ear organs are embedded in agarose, then subjected to a mechanical disruption that shears off bundles and leaves them in agarose blocks. With care in the dissection and in clean-up of the isolated bundles, contamination from cell bodies can be kept to a minimum. Isolated bundles can be analyzed by a variety of techniques, including immunocytochemistry, SDS-PAGE, immunoblotting, and mass spectrometry.",
keywords = "Agarose, Auditory, Hair bundles, Mass spectrometry, Mechanotransduction, Vestibular",
author = "Shin, {Jung Bum} and James Pagana and Gillespie, {Peter G.}",
year = "2009",
doi = "10.1007/978-1-59745-523-7_14",
language = "English (US)",
isbn = "9781934115626",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "241--255",
booktitle = "Auditory and Vestibular Research",
}