Abstract
Adeno-associated viruses (AAVs) are leading candidate vectors for gene-therapy applications. The AAV-3b capsid is closely related to the well characterized AAV-2 capsid (87% identity), but sequence and presumably structural differences lead to distinct cell-entry and immune-recognition properties. In an effort to understand these differences and to perhaps harness them, diffraction-quality crystals of purified infectious AAV-3b particles have been grown and several partial diffraction data sets have been recorded. The crystals displayed varying levels of merohedral twinning that in earlier times would have rendered them unsuitable for structure determination, but here is shown to be a tractable complication.
Original language | English (US) |
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Pages (from-to) | 177-183 |
Number of pages | 7 |
Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
Volume | 65 |
Issue number | 2 |
DOIs | |
State | Published - 2009 |
Externally published | Yes |
Keywords
- Adeno-associated viruses
- Gene therapy
- Parvoviruses
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics