Twinned crystals of adeno-associated virus serotype 3b prove suitable for structural studies

Thomas F. Lerch, Qing Xie, Heather M. Ongley, Joan Hare, Michael S. Chapman

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Adeno-associated viruses (AAVs) are leading candidate vectors for gene-therapy applications. The AAV-3b capsid is closely related to the well characterized AAV-2 capsid (87% identity), but sequence and presumably structural differences lead to distinct cell-entry and immune-recognition properties. In an effort to understand these differences and to perhaps harness them, diffraction-quality crystals of purified infectious AAV-3b particles have been grown and several partial diffraction data sets have been recorded. The crystals displayed varying levels of merohedral twinning that in earlier times would have rendered them unsuitable for structure determination, but here is shown to be a tractable complication.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume65
Issue number2
DOIs
StatePublished - 2009

Keywords

  • Adeno-associated viruses
  • Gene therapy
  • Parvoviruses

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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