TY - JOUR
T1 - Tunability and noise dependence in differentiation dynamics
AU - Süel, Gürol M.
AU - Kulkarni, Rajan P.
AU - Dworkin, Jonathan
AU - Garcia-Ojalvo, Jordi
AU - Elowitz, Michael B.
PY - 2007/3/23
Y1 - 2007/3/23
N2 - The dynamic process of differentiation depends on the architecture, quantitative parameters, and noise of underlying genetic circuits. However, it remains unclear how these elements combine to control cellular behavior. We analyzed the probabilistic and transient differentiation of Bacillus subtilis cells into the state of competence. A few key parameters independently tuned the frequency of initiation and the duration of competence episodes and allowed the circuit to access different dynamic regimes, including oscillation. Altering circuit architecture showed that the duration of competence events can be made more precise. We used an experimental method to reduce global cellular noise and showed that noise levels are correlated with frequency of differentiation events. Together, the data reveal a noise-dependent circuit that is remarkably resilient and tunable in terms of its dynamic behavior.
AB - The dynamic process of differentiation depends on the architecture, quantitative parameters, and noise of underlying genetic circuits. However, it remains unclear how these elements combine to control cellular behavior. We analyzed the probabilistic and transient differentiation of Bacillus subtilis cells into the state of competence. A few key parameters independently tuned the frequency of initiation and the duration of competence episodes and allowed the circuit to access different dynamic regimes, including oscillation. Altering circuit architecture showed that the duration of competence events can be made more precise. We used an experimental method to reduce global cellular noise and showed that noise levels are correlated with frequency of differentiation events. Together, the data reveal a noise-dependent circuit that is remarkably resilient and tunable in terms of its dynamic behavior.
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U2 - 10.1126/science.1137455
DO - 10.1126/science.1137455
M3 - Article
C2 - 17379809
AN - SCOPUS:34047118521
SN - 0036-8075
VL - 315
SP - 1716
EP - 1719
JO - Science
JF - Science
IS - 5819
ER -