Tumor necrosis factor alpha leads to increased cell surface expression of CXCR4 in SK-N-MC cells

Kevin Rostasy, Gullu Gorgun, Yelena Kleyner, Anthony Garcia, Michael Kramer, Suzanne M. Melanson, Jean Marie Mathys, Constantin Yiannoutsos, Paul R. Skolnik, Bradford A. Navia

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Both host and viral factors play an important role in the pathogenesis of human immunodeficiency virus (HIV)-associated bran injury. In this study, the authors examined the interactions between tumor necrosis factor (TNF)-α, CXCR4, the alpha chemokine receptor, and three HIV isolates, including the T-tropic viruses, HIV-1MN and HIV-1IIIB, and the dual tropic virus, HIV-189.6. The authors show by flow cytometry that treatment of differentiated SK-N-MC cells with TNF-α induces a significant increase in the cell surface expression of CXCR4 in a time- and dose-dependent manner. The effect is partly regulated at the level of transcription. To assess the biological significance of this finding, we show that TNF-α potentiates the ability of the above mentioned HIV isolates to induce neuronal apoptosis and that the effect is significantly reduced by pretreating cells with monoclonal antibodies to either CXCR4 and TNF-α. Together these results suggest that TNF-α may render neuronal cells vulnerable to the apoptotic effects of HIV by increasing the cell surface expression of CXCR4 and thus identify another mechanism by which TNF-α contributes to the pathogenesis of HIV-associated brain injury.

Original languageEnglish (US)
Pages (from-to)247-255
Number of pages9
JournalJournal of NeuroVirology
Volume11
Issue number3
DOIs
StatePublished - Jul 2005
Externally publishedYes

Fingerprint

Tumor Necrosis Factor-alpha
HIV
Viruses
CXC Chemokines
Chemokine Receptors
Brain Injuries
Flow Cytometry
Monoclonal Antibodies
Apoptosis
Wounds and Injuries

Keywords

  • AIDS
  • Chemokine receptors
  • CXCR4
  • HIV dementia
  • TNF-α

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology

Cite this

Rostasy, K., Gorgun, G., Kleyner, Y., Garcia, A., Kramer, M., Melanson, S. M., ... Navia, B. A. (2005). Tumor necrosis factor alpha leads to increased cell surface expression of CXCR4 in SK-N-MC cells. Journal of NeuroVirology, 11(3), 247-255. https://doi.org/10.1080/13550280590952763

Tumor necrosis factor alpha leads to increased cell surface expression of CXCR4 in SK-N-MC cells. / Rostasy, Kevin; Gorgun, Gullu; Kleyner, Yelena; Garcia, Anthony; Kramer, Michael; Melanson, Suzanne M.; Mathys, Jean Marie; Yiannoutsos, Constantin; Skolnik, Paul R.; Navia, Bradford A.

In: Journal of NeuroVirology, Vol. 11, No. 3, 07.2005, p. 247-255.

Research output: Contribution to journalArticle

Rostasy, K, Gorgun, G, Kleyner, Y, Garcia, A, Kramer, M, Melanson, SM, Mathys, JM, Yiannoutsos, C, Skolnik, PR & Navia, BA 2005, 'Tumor necrosis factor alpha leads to increased cell surface expression of CXCR4 in SK-N-MC cells', Journal of NeuroVirology, vol. 11, no. 3, pp. 247-255. https://doi.org/10.1080/13550280590952763
Rostasy, Kevin ; Gorgun, Gullu ; Kleyner, Yelena ; Garcia, Anthony ; Kramer, Michael ; Melanson, Suzanne M. ; Mathys, Jean Marie ; Yiannoutsos, Constantin ; Skolnik, Paul R. ; Navia, Bradford A. / Tumor necrosis factor alpha leads to increased cell surface expression of CXCR4 in SK-N-MC cells. In: Journal of NeuroVirology. 2005 ; Vol. 11, No. 3. pp. 247-255.
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