Tumor necrosis factor-α is not essential in endotoxin induced eye inflammation: Studies in cytokine receptor deficient mice

James (Jim) Rosenbaum, Young Bok Han, Jong Moon Park, Michael Kennedy, Stephen Planck

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objective. Anterior uveitis frequently occurs in association with specific systemic inflammatory diseases. Interleukin 1 (IL-1) and tumor necrosis factor-α (TNF-α) have been implicated in the pathogenesis of these diseases. We evaluate the need for these cytokines in a model of anterior uveitis. Methods. Endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I, TNF receptors p55 and p75, both IL-1R1 and TNFR p55, or controls. Eyes were harvested after 24 h for histology and IL-6 bioassays or after 3 h for reverse transcriptase-polymerase chain reaction analysis of mRNA for specific cytokines or enzymes. Results. No significant difference in the number of infiltrating cells was found in TNFR p55/p75 deficient mice compared to controls in any of 4 separate experiments or in the combined data (p = 0.8). The number of infiltrating cells was significantly reduced in 2 of 4 experiments with IL-1R1 deficient mice (p <0.001 based on combined data from 4 studies). IL-1R1/TNFR p55 deficient mice had a reduction in infiltrating cells in 2 of 3 experiments (p <0.001 based on combined data from all studies). IL-6 levels were not significantly reduced in either of 2 experiments with TNFR p55/p75 deficient mice, but were reduced in one of 2 experiments with IL-1R1(-/-) mice (p = 0.02) and in one experiment with IL- 1R1/TNFR p55 deficient mice (p = 0.01). In response to endotoxin, all 3 receptor deficient lines increased mRNA levels for IL-1-α, IL-10, TNF-α, IL-1 receptor antagonist, and inducible nitric oxide synthase. Conclusions. IL-1 appears to have a more pivotal role in endotoxin induced uveitis than TNF-α, although neither cytokine is essential. Deletion of receptors for both cytokines has the most consistent effect, which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant.

Original languageEnglish (US)
Pages (from-to)2408-2416
Number of pages9
JournalJournal of Rheumatology
Volume25
Issue number12
StatePublished - Dec 1998

Fingerprint

Cytokine Receptors
Endotoxins
Tumor Necrosis Factor-alpha
Inflammation
Interleukin-1
Receptors, Tumor Necrosis Factor, Type II
Cytokines
Anterior Uveitis
Interleukin-6
Interleukin-1 Type I Receptors
Cell Count
Messenger RNA
Interleukin-1 Receptors
Uveitis
Nitric Oxide Synthase Type II
Reverse Transcriptase Polymerase Chain Reaction
Biological Assay
Interleukin-10
Histology
Enzymes

Keywords

  • Interleukin 1
  • Knockout mice
  • Lipopolysaccharides
  • Tumor necrosis factor
  • Uveitis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

Tumor necrosis factor-α is not essential in endotoxin induced eye inflammation : Studies in cytokine receptor deficient mice. / Rosenbaum, James (Jim); Han, Young Bok; Park, Jong Moon; Kennedy, Michael; Planck, Stephen.

In: Journal of Rheumatology, Vol. 25, No. 12, 12.1998, p. 2408-2416.

Research output: Contribution to journalArticle

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abstract = "Objective. Anterior uveitis frequently occurs in association with specific systemic inflammatory diseases. Interleukin 1 (IL-1) and tumor necrosis factor-α (TNF-α) have been implicated in the pathogenesis of these diseases. We evaluate the need for these cytokines in a model of anterior uveitis. Methods. Endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I, TNF receptors p55 and p75, both IL-1R1 and TNFR p55, or controls. Eyes were harvested after 24 h for histology and IL-6 bioassays or after 3 h for reverse transcriptase-polymerase chain reaction analysis of mRNA for specific cytokines or enzymes. Results. No significant difference in the number of infiltrating cells was found in TNFR p55/p75 deficient mice compared to controls in any of 4 separate experiments or in the combined data (p = 0.8). The number of infiltrating cells was significantly reduced in 2 of 4 experiments with IL-1R1 deficient mice (p <0.001 based on combined data from 4 studies). IL-1R1/TNFR p55 deficient mice had a reduction in infiltrating cells in 2 of 3 experiments (p <0.001 based on combined data from all studies). IL-6 levels were not significantly reduced in either of 2 experiments with TNFR p55/p75 deficient mice, but were reduced in one of 2 experiments with IL-1R1(-/-) mice (p = 0.02) and in one experiment with IL- 1R1/TNFR p55 deficient mice (p = 0.01). In response to endotoxin, all 3 receptor deficient lines increased mRNA levels for IL-1-α, IL-10, TNF-α, IL-1 receptor antagonist, and inducible nitric oxide synthase. Conclusions. IL-1 appears to have a more pivotal role in endotoxin induced uveitis than TNF-α, although neither cytokine is essential. Deletion of receptors for both cytokines has the most consistent effect, which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant.",
keywords = "Interleukin 1, Knockout mice, Lipopolysaccharides, Tumor necrosis factor, Uveitis",
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T1 - Tumor necrosis factor-α is not essential in endotoxin induced eye inflammation

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AU - Rosenbaum, James (Jim)

AU - Han, Young Bok

AU - Park, Jong Moon

AU - Kennedy, Michael

AU - Planck, Stephen

PY - 1998/12

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N2 - Objective. Anterior uveitis frequently occurs in association with specific systemic inflammatory diseases. Interleukin 1 (IL-1) and tumor necrosis factor-α (TNF-α) have been implicated in the pathogenesis of these diseases. We evaluate the need for these cytokines in a model of anterior uveitis. Methods. Endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I, TNF receptors p55 and p75, both IL-1R1 and TNFR p55, or controls. Eyes were harvested after 24 h for histology and IL-6 bioassays or after 3 h for reverse transcriptase-polymerase chain reaction analysis of mRNA for specific cytokines or enzymes. Results. No significant difference in the number of infiltrating cells was found in TNFR p55/p75 deficient mice compared to controls in any of 4 separate experiments or in the combined data (p = 0.8). The number of infiltrating cells was significantly reduced in 2 of 4 experiments with IL-1R1 deficient mice (p <0.001 based on combined data from 4 studies). IL-1R1/TNFR p55 deficient mice had a reduction in infiltrating cells in 2 of 3 experiments (p <0.001 based on combined data from all studies). IL-6 levels were not significantly reduced in either of 2 experiments with TNFR p55/p75 deficient mice, but were reduced in one of 2 experiments with IL-1R1(-/-) mice (p = 0.02) and in one experiment with IL- 1R1/TNFR p55 deficient mice (p = 0.01). In response to endotoxin, all 3 receptor deficient lines increased mRNA levels for IL-1-α, IL-10, TNF-α, IL-1 receptor antagonist, and inducible nitric oxide synthase. Conclusions. IL-1 appears to have a more pivotal role in endotoxin induced uveitis than TNF-α, although neither cytokine is essential. Deletion of receptors for both cytokines has the most consistent effect, which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant.

AB - Objective. Anterior uveitis frequently occurs in association with specific systemic inflammatory diseases. Interleukin 1 (IL-1) and tumor necrosis factor-α (TNF-α) have been implicated in the pathogenesis of these diseases. We evaluate the need for these cytokines in a model of anterior uveitis. Methods. Endotoxin was injected into the vitreous of mice deficient in IL-1 receptor type I, TNF receptors p55 and p75, both IL-1R1 and TNFR p55, or controls. Eyes were harvested after 24 h for histology and IL-6 bioassays or after 3 h for reverse transcriptase-polymerase chain reaction analysis of mRNA for specific cytokines or enzymes. Results. No significant difference in the number of infiltrating cells was found in TNFR p55/p75 deficient mice compared to controls in any of 4 separate experiments or in the combined data (p = 0.8). The number of infiltrating cells was significantly reduced in 2 of 4 experiments with IL-1R1 deficient mice (p <0.001 based on combined data from 4 studies). IL-1R1/TNFR p55 deficient mice had a reduction in infiltrating cells in 2 of 3 experiments (p <0.001 based on combined data from all studies). IL-6 levels were not significantly reduced in either of 2 experiments with TNFR p55/p75 deficient mice, but were reduced in one of 2 experiments with IL-1R1(-/-) mice (p = 0.02) and in one experiment with IL- 1R1/TNFR p55 deficient mice (p = 0.01). In response to endotoxin, all 3 receptor deficient lines increased mRNA levels for IL-1-α, IL-10, TNF-α, IL-1 receptor antagonist, and inducible nitric oxide synthase. Conclusions. IL-1 appears to have a more pivotal role in endotoxin induced uveitis than TNF-α, although neither cytokine is essential. Deletion of receptors for both cytokines has the most consistent effect, which is in accord with the hypothesis that these cytokines are, at least in part, functionally redundant.

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