Tumor necrosis factor-α increases brain-derived neurotrophic factor expression in trigeminal ganglion neurons in an activity-dependent manner

E. Bałkowiec-Iskra, A. Vermehren-Schmaedick, Agnieszka Balkowiec

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Many chronic trigeminal pain conditions, such as migraine or temporo-mandibular disorders, are associated with inflammation within peripheral endings of trigeminal ganglion (TG) sensory neurons. A critical role in mechanisms of neuroinflammation is attributed to proinflammatory cytokines, such as interleukin-1β and tumor necrosis factor-α (TNFα) that also contribute to mechanisms of persistent neuropathic pain resulting from nerve injury. However, the mechanisms of cytokine-mediated synaptic plasticity and nociceptor sensitization are not completely understood. In the present study, we examined the effects of TNFα on neuronal expression of brain-derived neurotrophic factor (BDNF), whose role in synaptic plasticity and sensitization of nociceptive pathways is well documented. We show that 4- and 24-h treatment with TNFα increases BDNF mRNA and protein, respectively, in neuron-enriched dissociated cultures of rat TG. TNFα increases the phosphorylated form of the cyclic AMP-responsive element binding protein (CREB), a transcription factor involved in regulation of BDNF expression in neurons, and activates transcription of BDNF exon IV (former exon III) and, to a lesser extent, exon VI (former exon IV), but not exon I. TNFα-mediated increase in BDNF expression is accompanied by increase in calcitonin gene-related peptide (CGRP), which is consistent with previously published studies, and indicates that both peptides are similarly regulated in TG neurons by inflammatory mediators. The effect of TNFα on BDNF expression is dependent on sodium influx through TTX-sensitive channels and on p38-mitogen-activated protein kinase. Moreover, electrical stimulation and forskolin, known to increase intracellular cAMP, potentiate the TNFα-mediated upregulation of BDNF expression. This study provides new evidence for a direct action of proinflammatory cytokines on TG primary sensory neurons, and reveals a mechanism through which TNFα stimulates de novo synthesis of BDNF in these neurons. Thus, TNFα should be considered in mechanisms of BDNF-dependent neuronal plasticity.

Original languageEnglish (US)
Pages (from-to)322-333
Number of pages12
JournalNeuroscience
Volume180
DOIs
StatePublished - Apr 28 2011

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Trigeminal Ganglion
Brain-Derived Neurotrophic Factor
Tumor Necrosis Factor-alpha
Neurons
Exons
Neuronal Plasticity
Sensory Receptor Cells
Cytokines
Nociceptors
Calcitonin Gene-Related Peptide
Nerve Growth Factors
Neuralgia
p38 Mitogen-Activated Protein Kinases
Colforsin
Migraine Disorders
Interleukin-1
Chronic Pain
Cyclic AMP
Electric Stimulation
Carrier Proteins

Keywords

  • Activity
  • BDNF
  • Inflammation
  • P38-MAPK
  • TNFα
  • Trigeminal

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Tumor necrosis factor-α increases brain-derived neurotrophic factor expression in trigeminal ganglion neurons in an activity-dependent manner. / Bałkowiec-Iskra, E.; Vermehren-Schmaedick, A.; Balkowiec, Agnieszka.

In: Neuroscience, Vol. 180, 28.04.2011, p. 322-333.

Research output: Contribution to journalArticle

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