Triggered release of hydrophilic agents from plasmologen liposomes using visible light or acid

Valerie Anderson, David H. Thompson

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Triggered release from liposomes composed of semi-synthetic 1-alk-1′-enyl-2-acyl-sn-glycero-3-phosphocholine (plasmologen) lipids has been demonstrated using either aerobic visible illumination or low pH to induce leakage. The photodynamic release system consists of three functional components: (1) small (<1000 A ̊) unilamellar plasmologen vesicles (SUVs) containing encapsulated glucose, (2) oxygen and (3) zinc phthalocyanine (ZnPc) incorporated within the hydrophobic region of the SUV membrane. Irradiation (λ > 640 nm) at 37°C of air-saturated 1-alk-1′-enyl-2-palmitoyl-sn-glycero-3- phosphocholine (PlasPPC)/ 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) (8:1 mol/mol) liposomes at physiologically relevant temperatures results in glucose release rates that are twice those of the corresponding dark control. Photolysis of argon-saturated PlasPPC/DPPC liposomes or of identical vesicles lacking either ZnPc or the plasmalogen vinyl ether bond exhibit glucose release curves which are indistinguishable from the dark control. Irradiation under identical conditions, but in the presence of 100 mM sodium azide, also results in no increased rate of glucose release above that of the dark control. TLC analysis indicates that oxidized lipid species are produced only in air-saturated, irradiated plasmologen liposomes. The acid lability of the plasmologen vinyl ether linkage has also been used to trigger release of entrapped calcein. At pH 4.2, the release rate at 37°C is increased 4-fold over rates observed at pH 8. TLC analysis indicates formation of a lysoplamalogen product. Taken together, these results indicate that both photodynamic and acid triggering can be used to increase plasmalogen liposome permeability and suggest that these liposomes are potentially useful for drug delivery applications.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalBBA - Biomembranes
Volume1109
Issue number1
DOIs
Publication statusPublished - Aug 10 1992

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Keywords

  • Drug delivery
  • Liposome
  • Plasmalogen
  • Triggered release
  • Zine phthalocyanine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

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