Abstract
Background: Margination occurs when blood borne particles attach to the vessel wall. Triacylglycerol-rich lipoprotein (TRL) particles marginale when they bind to endothelial lipoprotein lipase (LpL). Objective: This study was undertaken to determine whether TRL margination reflects in vivo LpL activity and whether n-3 fatty acids affect fasting and fed TRL margination. Design: Healthy subjects (n = 33) began with a 4-wk, placebo (olive oil; 4 g/d) run-in period and were then randomly assigned to 4 wk of treatment with 4 g/d of ethyl esters of either safflower oil (SAF; control), eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA). Margination volume (MV) was calculated by subtracting true from apparent plasma volume. Results: MVs were 3 times as great during the fasting state as during the fed state (P <0.0001). In both the fasting and the fed states, MV was significantly correlated with plasma triacylglycerol and TRL half-lives. In the fed state, MV was also correlated with preheparin LpL, whereas in the fasting state it was not. There was no significant correlation between preheparin LpL and postheparin LpL in the fasting state. Relative to SAF, EPA and DHA supplementation resulted in higher MVs by 64% and 53% (both P <0.001), respectively, in the fasting state, without significantly reducing fasting triacylglycerol concentrations. In the fed state, DHA doubled the MV (P <0.05), whereas EPA had no significant effect. Conclusions: The correlations between MV and TRL half-lives and preheparin LpL suggest that MV could be a reflection of whole-body LpL binding capacity. The increases in MVs with EPA and DHA supplementation suggest that these fatty acids may increase the amount of endothelial-bound LpL or its affinity for TRL.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-50 |
| Number of pages | 6 |
| Journal | American Journal of Clinical Nutrition |
| Volume | 80 |
| Issue number | 1 |
| State | Published - Jul 2004 |
| Externally published | Yes |
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Keywords
- Chylomicrons
- Docosahexaenoic acid
- Eicosapentaenoic acid
- Lipoprotein lipase
- Margination
- N-3 fatty acids
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science
Cite this
Triacylglycerol-rich lipoprotein margination : A potential surrogate for whole-body lipoprotein lipase activity and effects of eicosapentaenoic and docosahexaenoic acids. / Park, Yongsoon; Jones, Philip G.; Harris, William.
In: American Journal of Clinical Nutrition, Vol. 80, No. 1, 07.2004, p. 45-50.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Triacylglycerol-rich lipoprotein margination
T2 - A potential surrogate for whole-body lipoprotein lipase activity and effects of eicosapentaenoic and docosahexaenoic acids
AU - Park, Yongsoon
AU - Jones, Philip G.
AU - Harris, William
PY - 2004/7
Y1 - 2004/7
N2 - Background: Margination occurs when blood borne particles attach to the vessel wall. Triacylglycerol-rich lipoprotein (TRL) particles marginale when they bind to endothelial lipoprotein lipase (LpL). Objective: This study was undertaken to determine whether TRL margination reflects in vivo LpL activity and whether n-3 fatty acids affect fasting and fed TRL margination. Design: Healthy subjects (n = 33) began with a 4-wk, placebo (olive oil; 4 g/d) run-in period and were then randomly assigned to 4 wk of treatment with 4 g/d of ethyl esters of either safflower oil (SAF; control), eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA). Margination volume (MV) was calculated by subtracting true from apparent plasma volume. Results: MVs were 3 times as great during the fasting state as during the fed state (P <0.0001). In both the fasting and the fed states, MV was significantly correlated with plasma triacylglycerol and TRL half-lives. In the fed state, MV was also correlated with preheparin LpL, whereas in the fasting state it was not. There was no significant correlation between preheparin LpL and postheparin LpL in the fasting state. Relative to SAF, EPA and DHA supplementation resulted in higher MVs by 64% and 53% (both P <0.001), respectively, in the fasting state, without significantly reducing fasting triacylglycerol concentrations. In the fed state, DHA doubled the MV (P <0.05), whereas EPA had no significant effect. Conclusions: The correlations between MV and TRL half-lives and preheparin LpL suggest that MV could be a reflection of whole-body LpL binding capacity. The increases in MVs with EPA and DHA supplementation suggest that these fatty acids may increase the amount of endothelial-bound LpL or its affinity for TRL.
AB - Background: Margination occurs when blood borne particles attach to the vessel wall. Triacylglycerol-rich lipoprotein (TRL) particles marginale when they bind to endothelial lipoprotein lipase (LpL). Objective: This study was undertaken to determine whether TRL margination reflects in vivo LpL activity and whether n-3 fatty acids affect fasting and fed TRL margination. Design: Healthy subjects (n = 33) began with a 4-wk, placebo (olive oil; 4 g/d) run-in period and were then randomly assigned to 4 wk of treatment with 4 g/d of ethyl esters of either safflower oil (SAF; control), eicosapentaenoic acid (EPA), or docosahexaenoic acid (DHA). Margination volume (MV) was calculated by subtracting true from apparent plasma volume. Results: MVs were 3 times as great during the fasting state as during the fed state (P <0.0001). In both the fasting and the fed states, MV was significantly correlated with plasma triacylglycerol and TRL half-lives. In the fed state, MV was also correlated with preheparin LpL, whereas in the fasting state it was not. There was no significant correlation between preheparin LpL and postheparin LpL in the fasting state. Relative to SAF, EPA and DHA supplementation resulted in higher MVs by 64% and 53% (both P <0.001), respectively, in the fasting state, without significantly reducing fasting triacylglycerol concentrations. In the fed state, DHA doubled the MV (P <0.05), whereas EPA had no significant effect. Conclusions: The correlations between MV and TRL half-lives and preheparin LpL suggest that MV could be a reflection of whole-body LpL binding capacity. The increases in MVs with EPA and DHA supplementation suggest that these fatty acids may increase the amount of endothelial-bound LpL or its affinity for TRL.
KW - Chylomicrons
KW - Docosahexaenoic acid
KW - Eicosapentaenoic acid
KW - Lipoprotein lipase
KW - Margination
KW - N-3 fatty acids
UR - http://www.scopus.com/inward/record.url?scp=3042740696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042740696&partnerID=8YFLogxK
M3 - Article
C2 - 15213026
AN - SCOPUS:3042740696
VL - 80
SP - 45
EP - 50
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
SN - 0002-9165
IS - 1
ER -