Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions

Anna Bar, Nicholas Snavely, Nathaniel Chen, Steven Jacques, Daniel S. Gareau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Screening cancer in excision margins may be done with confocal microscopy to save time and cost over the gold standard histopathology (H&E). However, diagnostic accuracy requires sufficient contrast. Reflectance mode enables detection of large (>500um) nodular tumors. Enhanced nuclear contrast with acridine orange fluorescence mode additionally enables detection of tiny (<50um) basal cell carcinomas. Here, we present a novel combination of three modes to detect squamous cell carcinoma (SCC). Accurate screening of SCC requires eosin fluorescence, reflectance and acridine orange fluorescence to enable contrast for cytoplasm, collagen and nuclei respectively. Combining these signals replicates H&E for rapid clinical translation.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems X
DOIs
StatePublished - Jun 12 2012
EventAdvanced Biomedical and Clinical Diagnostic Systems X - San Francisco, CA, United States
Duration: Jan 22 2012Jan 24 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8214
ISSN (Print)1605-7422

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems X
CountryUnited States
CitySan Francisco, CA
Period1/22/121/24/12

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Bar, A., Snavely, N., Chen, N., Jacques, S., & Gareau, D. S. (2012). Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions. In Advanced Biomedical and Clinical Diagnostic Systems X [821416] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8214). https://doi.org/10.1117/12.909902