Transposon Tn5 mutagenesis of Brucella abortus

L. D. Smith, Fred Heffron

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.

Original languageEnglish (US)
Pages (from-to)2774-2776
Number of pages3
JournalInfection and Immunity
Volume55
Issue number11
StatePublished - 1987
Externally publishedYes

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Brucella abortus
Kanamycin
Mutagenesis
Chromosomes
Brucella
Bacteriophages
Plasmids
Mutation

ASJC Scopus subject areas

  • Immunology

Cite this

Smith, L. D., & Heffron, F. (1987). Transposon Tn5 mutagenesis of Brucella abortus. Infection and Immunity, 55(11), 2774-2776.

Transposon Tn5 mutagenesis of Brucella abortus. / Smith, L. D.; Heffron, Fred.

In: Infection and Immunity, Vol. 55, No. 11, 1987, p. 2774-2776.

Research output: Contribution to journalArticle

Smith, LD & Heffron, F 1987, 'Transposon Tn5 mutagenesis of Brucella abortus', Infection and Immunity, vol. 55, no. 11, pp. 2774-2776.
Smith LD, Heffron F. Transposon Tn5 mutagenesis of Brucella abortus. Infection and Immunity. 1987;55(11):2774-2776.
Smith, L. D. ; Heffron, Fred. / Transposon Tn5 mutagenesis of Brucella abortus. In: Infection and Immunity. 1987 ; Vol. 55, No. 11. pp. 2774-2776.
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