Transposon Tn5 mutagenesis of Brucella abortus

L. D. Smith, F. Heffron

    Research output: Contribution to journalArticlepeer-review

    10 Scopus citations


    We demonstrate that transposon-mediated mutagenesis can be used to construct mutations in the pathogen Brucella abortus. We have used both a plasmid and a bacteriophage to introduce either Tn5 or Tn5 lac into the Brucella chromosome. B. abortus is naturally sensitive to kanamycin. We have selected 22 independent kanamycin-resistant colonies in strain US-19 and 19 colonies in strain 2308. We have demonstrated by Southern hybridization that Tn5 was inserted into the chromosome.

    Original languageEnglish (US)
    Pages (from-to)2774-2776
    Number of pages3
    JournalInfection and Immunity
    Issue number11
    StatePublished - 1987

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases


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