Transport of methionine in Trypanosoma brucei brucei

Marie Pierre Hasne, Michael P. Barrett

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

African trypanosomes live free in the bloodstream and central nervous system of mammalian hosts and also within the midgut of the tsetse fly vectors which transmit them. The parasite plasma membrane represents the interface between both hosts and parasite, and trypanosomes accumulate many essential metabolites via specific transport processes. L-Methionine uptake by procyclic and bloodstream forms of Trypanosoma brucei has been measured and shown to be mediated by a transporter presenting similar characteristics in both forms of the parasite. The carrier shows, in both forms, a relatively high affinity for methionine (Km ca. 30 μM). The effect of inhibitors of ion gradients across the membrane indicated that the uptake process is likely to be dependent upon a proton motive force. Various methionine analogues were tested against the transporter and these have demonstrated that the recognition depends on the motif common to all amino acids, and an electronegative group at the position of the sulphur atom separated from the α-carbon atom by a two carbon spacer.

Original languageEnglish (US)
Pages (from-to)299-307
Number of pages9
JournalMolecular and Biochemical Parasitology
Volume111
Issue number2
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Trypanosoma brucei brucei
Methionine
Parasites
Trypanosomiasis
Carbon
Tsetse Flies
Proton-Motive Force
Sulfur
Central Nervous System
Cell Membrane
Ions
Amino Acids
Membranes

Keywords

  • Amino acid transport
  • Methionine
  • Trypanosoma brucei

ASJC Scopus subject areas

  • Molecular Biology
  • Parasitology

Cite this

Transport of methionine in Trypanosoma brucei brucei. / Pierre Hasne, Marie; Barrett, Michael P.

In: Molecular and Biochemical Parasitology, Vol. 111, No. 2, 2000, p. 299-307.

Research output: Contribution to journalArticle

Pierre Hasne, Marie ; Barrett, Michael P. / Transport of methionine in Trypanosoma brucei brucei. In: Molecular and Biochemical Parasitology. 2000 ; Vol. 111, No. 2. pp. 299-307.
@article{0d087299177846f0bd65debfc07d856f,
title = "Transport of methionine in Trypanosoma brucei brucei",
abstract = "African trypanosomes live free in the bloodstream and central nervous system of mammalian hosts and also within the midgut of the tsetse fly vectors which transmit them. The parasite plasma membrane represents the interface between both hosts and parasite, and trypanosomes accumulate many essential metabolites via specific transport processes. L-Methionine uptake by procyclic and bloodstream forms of Trypanosoma brucei has been measured and shown to be mediated by a transporter presenting similar characteristics in both forms of the parasite. The carrier shows, in both forms, a relatively high affinity for methionine (Km ca. 30 μM). The effect of inhibitors of ion gradients across the membrane indicated that the uptake process is likely to be dependent upon a proton motive force. Various methionine analogues were tested against the transporter and these have demonstrated that the recognition depends on the motif common to all amino acids, and an electronegative group at the position of the sulphur atom separated from the α-carbon atom by a two carbon spacer.",
keywords = "Amino acid transport, Methionine, Trypanosoma brucei",
author = "{Pierre Hasne}, Marie and Barrett, {Michael P.}",
year = "2000",
doi = "10.1016/S0166-6851(00)00321-2",
language = "English (US)",
volume = "111",
pages = "299--307",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Transport of methionine in Trypanosoma brucei brucei

AU - Pierre Hasne, Marie

AU - Barrett, Michael P.

PY - 2000

Y1 - 2000

N2 - African trypanosomes live free in the bloodstream and central nervous system of mammalian hosts and also within the midgut of the tsetse fly vectors which transmit them. The parasite plasma membrane represents the interface between both hosts and parasite, and trypanosomes accumulate many essential metabolites via specific transport processes. L-Methionine uptake by procyclic and bloodstream forms of Trypanosoma brucei has been measured and shown to be mediated by a transporter presenting similar characteristics in both forms of the parasite. The carrier shows, in both forms, a relatively high affinity for methionine (Km ca. 30 μM). The effect of inhibitors of ion gradients across the membrane indicated that the uptake process is likely to be dependent upon a proton motive force. Various methionine analogues were tested against the transporter and these have demonstrated that the recognition depends on the motif common to all amino acids, and an electronegative group at the position of the sulphur atom separated from the α-carbon atom by a two carbon spacer.

AB - African trypanosomes live free in the bloodstream and central nervous system of mammalian hosts and also within the midgut of the tsetse fly vectors which transmit them. The parasite plasma membrane represents the interface between both hosts and parasite, and trypanosomes accumulate many essential metabolites via specific transport processes. L-Methionine uptake by procyclic and bloodstream forms of Trypanosoma brucei has been measured and shown to be mediated by a transporter presenting similar characteristics in both forms of the parasite. The carrier shows, in both forms, a relatively high affinity for methionine (Km ca. 30 μM). The effect of inhibitors of ion gradients across the membrane indicated that the uptake process is likely to be dependent upon a proton motive force. Various methionine analogues were tested against the transporter and these have demonstrated that the recognition depends on the motif common to all amino acids, and an electronegative group at the position of the sulphur atom separated from the α-carbon atom by a two carbon spacer.

KW - Amino acid transport

KW - Methionine

KW - Trypanosoma brucei

UR - http://www.scopus.com/inward/record.url?scp=0034494342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034494342&partnerID=8YFLogxK

U2 - 10.1016/S0166-6851(00)00321-2

DO - 10.1016/S0166-6851(00)00321-2

M3 - Article

C2 - 11163438

AN - SCOPUS:0034494342

VL - 111

SP - 299

EP - 307

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -