Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells

Alexis S. Bailey, Shuguang Jiang, Michael Afentoulis, Christina I. Baumann, David A. Schroeder, Susan Olson, Melissa Wong, William Fleming

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

During early embryogenesis, blood vessels and hematopoietic cells arise from a common precursor cell, the hemangioblast. Recent studies have identified endothelial progenitor cells in the peripheral blood, and there is accumulating evidence that a subset of these cells is derived from precursors in the bone marrow. Here we show that adult bone marrow-derived, phenotypically defined hematopoietic stem cells (c-kit+, Sca-1+, lineage -) give rise to functional endothelial cells. With the exception of the brain, donor-derived cells are rapidly integrated into blood vessels. Durably engrafted endothelial cells express CD31, produce von Willebrand factor, and take up low-density lipoprotein. Analysis of DNA content indicates that donor-derived endothelial cells are not the products of cell fusion. Self-renewal of stem cells with hematopoietic and endothelial cell potential was revealed by serial transplantation studies. The clonal origin of both hematopoietic and endothelial cell outcomes was established by the transfer of a single cell. These results suggest that adult bone marrow-derived hematopoietic stem cells may serve as a reservoir for endothelial cell progenitors.

Original languageEnglish (US)
Pages (from-to)13-19
Number of pages7
JournalBlood
Volume103
Issue number1
DOIs
StatePublished - Jan 1 2004

Fingerprint

Adult Stem Cells
Endothelial cells
Hematopoietic Stem Cells
Stem cells
Endothelial Cells
Bone Marrow
Blood Vessels
Bone
Hemangioblasts
Blood vessels
Cell Fusion
von Willebrand Factor
LDL Lipoproteins
Embryonic Development
Transplantation
Brain
Blood
Fusion reactions
DNA

ASJC Scopus subject areas

  • Hematology

Cite this

Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells. / Bailey, Alexis S.; Jiang, Shuguang; Afentoulis, Michael; Baumann, Christina I.; Schroeder, David A.; Olson, Susan; Wong, Melissa; Fleming, William.

In: Blood, Vol. 103, No. 1, 01.01.2004, p. 13-19.

Research output: Contribution to journalArticle

Bailey, Alexis S. ; Jiang, Shuguang ; Afentoulis, Michael ; Baumann, Christina I. ; Schroeder, David A. ; Olson, Susan ; Wong, Melissa ; Fleming, William. / Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells. In: Blood. 2004 ; Vol. 103, No. 1. pp. 13-19.
@article{60736bd48a864dab940b849bff54e477,
title = "Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells",
abstract = "During early embryogenesis, blood vessels and hematopoietic cells arise from a common precursor cell, the hemangioblast. Recent studies have identified endothelial progenitor cells in the peripheral blood, and there is accumulating evidence that a subset of these cells is derived from precursors in the bone marrow. Here we show that adult bone marrow-derived, phenotypically defined hematopoietic stem cells (c-kit+, Sca-1+, lineage -) give rise to functional endothelial cells. With the exception of the brain, donor-derived cells are rapidly integrated into blood vessels. Durably engrafted endothelial cells express CD31, produce von Willebrand factor, and take up low-density lipoprotein. Analysis of DNA content indicates that donor-derived endothelial cells are not the products of cell fusion. Self-renewal of stem cells with hematopoietic and endothelial cell potential was revealed by serial transplantation studies. The clonal origin of both hematopoietic and endothelial cell outcomes was established by the transfer of a single cell. These results suggest that adult bone marrow-derived hematopoietic stem cells may serve as a reservoir for endothelial cell progenitors.",
author = "Bailey, {Alexis S.} and Shuguang Jiang and Michael Afentoulis and Baumann, {Christina I.} and Schroeder, {David A.} and Susan Olson and Melissa Wong and William Fleming",
year = "2004",
month = "1",
day = "1",
doi = "10.1182/blood-2003-05-1684",
language = "English (US)",
volume = "103",
pages = "13--19",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Transplanted adult hematopoietic stems cells differentiate into functional endothelial cells

AU - Bailey, Alexis S.

AU - Jiang, Shuguang

AU - Afentoulis, Michael

AU - Baumann, Christina I.

AU - Schroeder, David A.

AU - Olson, Susan

AU - Wong, Melissa

AU - Fleming, William

PY - 2004/1/1

Y1 - 2004/1/1

N2 - During early embryogenesis, blood vessels and hematopoietic cells arise from a common precursor cell, the hemangioblast. Recent studies have identified endothelial progenitor cells in the peripheral blood, and there is accumulating evidence that a subset of these cells is derived from precursors in the bone marrow. Here we show that adult bone marrow-derived, phenotypically defined hematopoietic stem cells (c-kit+, Sca-1+, lineage -) give rise to functional endothelial cells. With the exception of the brain, donor-derived cells are rapidly integrated into blood vessels. Durably engrafted endothelial cells express CD31, produce von Willebrand factor, and take up low-density lipoprotein. Analysis of DNA content indicates that donor-derived endothelial cells are not the products of cell fusion. Self-renewal of stem cells with hematopoietic and endothelial cell potential was revealed by serial transplantation studies. The clonal origin of both hematopoietic and endothelial cell outcomes was established by the transfer of a single cell. These results suggest that adult bone marrow-derived hematopoietic stem cells may serve as a reservoir for endothelial cell progenitors.

AB - During early embryogenesis, blood vessels and hematopoietic cells arise from a common precursor cell, the hemangioblast. Recent studies have identified endothelial progenitor cells in the peripheral blood, and there is accumulating evidence that a subset of these cells is derived from precursors in the bone marrow. Here we show that adult bone marrow-derived, phenotypically defined hematopoietic stem cells (c-kit+, Sca-1+, lineage -) give rise to functional endothelial cells. With the exception of the brain, donor-derived cells are rapidly integrated into blood vessels. Durably engrafted endothelial cells express CD31, produce von Willebrand factor, and take up low-density lipoprotein. Analysis of DNA content indicates that donor-derived endothelial cells are not the products of cell fusion. Self-renewal of stem cells with hematopoietic and endothelial cell potential was revealed by serial transplantation studies. The clonal origin of both hematopoietic and endothelial cell outcomes was established by the transfer of a single cell. These results suggest that adult bone marrow-derived hematopoietic stem cells may serve as a reservoir for endothelial cell progenitors.

UR - http://www.scopus.com/inward/record.url?scp=0346966883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346966883&partnerID=8YFLogxK

U2 - 10.1182/blood-2003-05-1684

DO - 10.1182/blood-2003-05-1684

M3 - Article

VL - 103

SP - 13

EP - 19

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -