Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes

M. P. Kavanaugh, M. J. Christie, P. B. Osborne, A. E. Busch, Ke-Zhong Shen, Y. N P H Wu Seeburg, John Adelman, R. A. North

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Voltage-dependent K+ channels (RBK1, RBK2 and RGK5) were co-expressed in Xenopus oocytes with 5-hydroxytryptamine (5-HT2) receptors. K+ currents measured 2-4 days later were inhibited by 5-HT (100 nM-10 μM, 20-30 s application) by up to 90 %. The effect of 5-HT was mimicked by intracellular injection of Ins(1,4,5)P3. Increasing the Ca2+ concentration at the inner surface of excised membrane patches did not decrease the K+ current.

Original languageEnglish (US)
Pages (from-to)899-902
Number of pages4
JournalBiochemical Journal
Volume277
Issue number3
StatePublished - 1991

Fingerprint

Xenopus
Oocytes
Transmitters
Serotonin
Electric potential
Injections
Membranes
serotonin 5 receptor

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kavanaugh, M. P., Christie, M. J., Osborne, P. B., Busch, A. E., Shen, K-Z., Wu Seeburg, Y. N. P. H., ... North, R. A. (1991). Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes. Biochemical Journal, 277(3), 899-902.

Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes. / Kavanaugh, M. P.; Christie, M. J.; Osborne, P. B.; Busch, A. E.; Shen, Ke-Zhong; Wu Seeburg, Y. N P H; Adelman, John; North, R. A.

In: Biochemical Journal, Vol. 277, No. 3, 1991, p. 899-902.

Research output: Contribution to journalArticle

Kavanaugh, MP, Christie, MJ, Osborne, PB, Busch, AE, Shen, K-Z, Wu Seeburg, YNPH, Adelman, J & North, RA 1991, 'Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes', Biochemical Journal, vol. 277, no. 3, pp. 899-902.
Kavanaugh MP, Christie MJ, Osborne PB, Busch AE, Shen K-Z, Wu Seeburg YNPH et al. Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes. Biochemical Journal. 1991;277(3):899-902.
Kavanaugh, M. P. ; Christie, M. J. ; Osborne, P. B. ; Busch, A. E. ; Shen, Ke-Zhong ; Wu Seeburg, Y. N P H ; Adelman, John ; North, R. A. / Transmitter regulation of voltage-dependant K+ channels expressed in Xenopus oocytes. In: Biochemical Journal. 1991 ; Vol. 277, No. 3. pp. 899-902.
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