Transmission electron microscopy for zebrafish larvae and adult lateral line nerve

Rebecca L. Cunningham, Kelly Monk

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Transmission electron microscopy (TEM) enables visualization of the ultrastructure of the myelin sheath. Schwann cells on the posterior lateral line nerves and motor nerves can be imaged by TEM. Here, we detail the multiday processing of larval trunks and dissected posterior lateral line for TEM, as well as how to trim embedded samples, section, and stain grids for imaging.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages385-400
Number of pages16
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1739
ISSN (Print)1064-3745

Fingerprint

Zebrafish
Transmission Electron Microscopy
Larva
Schwann Cells
Myelin Sheath
Coloring Agents

Keywords

  • Adult zebrafish lateral line nerve
  • Lateral line nerve
  • Motor nerves
  • Myelin ultrastructure
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Cunningham, R. L., & Monk, K. (2018). Transmission electron microscopy for zebrafish larvae and adult lateral line nerve. In Methods in Molecular Biology (pp. 385-400). (Methods in Molecular Biology; Vol. 1739). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7649-2_26

Transmission electron microscopy for zebrafish larvae and adult lateral line nerve. / Cunningham, Rebecca L.; Monk, Kelly.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 385-400 (Methods in Molecular Biology; Vol. 1739).

Research output: Chapter in Book/Report/Conference proceedingChapter

Cunningham, RL & Monk, K 2018, Transmission electron microscopy for zebrafish larvae and adult lateral line nerve. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1739, Humana Press Inc., pp. 385-400. https://doi.org/10.1007/978-1-4939-7649-2_26
Cunningham RL, Monk K. Transmission electron microscopy for zebrafish larvae and adult lateral line nerve. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 385-400. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7649-2_26
Cunningham, Rebecca L. ; Monk, Kelly. / Transmission electron microscopy for zebrafish larvae and adult lateral line nerve. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 385-400 (Methods in Molecular Biology).
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