Transmembrane signalling associated with ganglioside-induced CD4 modulation

William J. Morrison, Halina Offner, Arthur A. Vandenbark

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Ganglioside (GM1) treatment of CD4+ human CEM ltmphoma cells stimulated transient phosphoinositide (PI) breakdown, production of inositol phosphates (IP), protein phosphorylation and rapid decrease of CD4 surface expression. A comparison between the actions of GM1 and other agents that affect these signal transduction pathways demonstrated a distinct mechanism for GM1-induced decrease of CD4. GM1 stimulated both phospholipase C activity and protein phosphorylation but had no effect on either cellular cAMP levels or tyrosine kinase activity. Phorbol myristate acetate (PMA) stimulated protein phosphorylation and caused a significant decrease in surface display of CD4. Both of these processes were blocked by pretreating cells with the protein kinase C (PKC) inhibitor H7. These results demonstrate that GM1 stimulates PI turnover and induces a rapid decrease of CD4 surface expression by processes that do not activate adenylate cyclase or tyrosine kinase. They further demonstrate that the mechanism for GM1-induced decrease of CD4 is distinct from the CD4 internalization processes mediated by PKC activity.

Original languageEnglish (US)
Pages (from-to)135-141
Number of pages7
JournalImmunopharmacology
Volume20
Issue number2
DOIs
StatePublished - Jan 1 1990

Keywords

  • CD4 modulation
  • Ganglioside
  • Signal transduction

ASJC Scopus subject areas

  • Pharmacology

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