Transmembrane helices 3 and 4 are involved in substrate recognition by the Na+/dicarboxylate cotransporter, NaDC1

Naomi Oshiro, Steven King, Ana M. Pajor

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The Na+/dicarboxylate cotransporters (NaDC1) from mouse (m) and rabbit (rb) differ in their ability to handle glutarate. Substrate-dependent inward currents, measured using two-electrode voltage clamp, were similar for glutarate and succinate in Xenopus oocytes expressing mNaDC1. In contrast, currents evoked by glutarate in rbNaDC1 were only about 5% of the succinate-dependent currents. To identify domains involved in glutarate transport, we constructed a series of chimeric transporters between mouse and rabbit NaDC1. Although residues found in multiple transmembrane helices (TM) participate in glutarate transport, the most important contribution is made by TM 3 and 4 and the associated loops. The R(M3-4) chimera, consisting of rbNaDC1 with substitution of TM 3-4 from mNaDC1, had a decreased K0.5 glutarate of 4 mM compared with 15 mM in wild-type rbNaDC1 without any effect on K0.5 glutarate. The chimeras were also characterized using dual-label competitive uptakes with 14C-glutarate and 3H-succinate to calculate the transport specificity ratio (TSR), a measure of relative catalytic efficiency with the two substrates. The TSR analysis provides evidence for functional coupling in the transition state between TM 3 and 4. We conclude that TM 3 and 4 contain amino acid residues that are important determinants of substrate specificity and catalytic efficiency in NaDC1.

Original languageEnglish (US)
Pages (from-to)2302-2310
Number of pages9
JournalBiochemistry
Volume45
Issue number7
DOIs
StatePublished - Feb 21 2006

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Glutarates
Substrates
Succinic Acid
Rabbits
Clamping devices
Substrate Specificity
Xenopus
Oocytes
Labels
Electrodes
Substitution reactions
Amino Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transmembrane helices 3 and 4 are involved in substrate recognition by the Na+/dicarboxylate cotransporter, NaDC1. / Oshiro, Naomi; King, Steven; Pajor, Ana M.

In: Biochemistry, Vol. 45, No. 7, 21.02.2006, p. 2302-2310.

Research output: Contribution to journalArticle

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