Translesion synthesis past acrolein-derived DNA adducts by human mitochondrial DNA polymerase γ

Rajesh Kasiviswanathan, Irina Minko, Robert (Stephen) Lloyd, William C. Copeland

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Acrolein, a mutagenic aldehyde, is produced endogenously by lipid peroxidation and exogenously by combustion of organic materials, including tobacco products. Acrolein reacts with DNA bases forming exocyclic DNA adducts, such as γ-hydroxy-1,N2-propano-2′-deoxyguanosine (γ-HOPdG) and γ-hydroxy-1,N6-propano-2′- deoxyadenosine (γ-HOPdA). The bulky γ-HOPdG adduct blocks DNA synthesis by replicative polymerases but can be bypassed by translesion synthesis polymerases in the nucleus. Although acrolein-induced adducts are likely to be formed and persist in mitochondrial DNA, animal cell mitochondria lack specialized translesion DNA synthesis polymerases to tolerate these lesions. Thus, it is important to understand how pol γ, the sole mitochondrial DNA polymerase in human cells, acts on acrolein-adducted DNA. To address this question, we investigated the ability of pol γ to bypass the minor groove γ-HOPdG and major groove γ-HOPdA adducts using single nucleotide incorporation and primer extension analyses. The efficiency of pol γ-catalyzed bypass of γ-HOPdG was low, and surprisingly, pol γ preferred to incorporate purine nucleotides opposite the adduct. Pol γ also exhibited ∼2-fold lower rates of excision of the misincorporated purine nucleotides opposite γ-HOPdG compared with the corresponding nucleotides opposite dG. Extension of primers from the termini opposite γ-HOPdG was accomplished only following error-prone purine nucleotide incorporation. However, pol γ preferentially incorporated dT opposite the γ-HOPdA adduct and efficiently extended primers from the correctly paired terminus, indicating that γ-HOPdA is probably nonmutagenic. In summary, our data suggest that acrolein-induced exocyclic DNA lesions can be bypassed by mitochondrial DNA polymerase but, in the case of the minor groove γ-HOPdG adduct, at the cost of unprecedented high mutation rates.

Original languageEnglish (US)
Pages (from-to)14247-14255
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number20
DOIs
StatePublished - May 17 2013

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Acrolein
DNA Adducts
DNA-Directed DNA Polymerase
Mitochondrial DNA
Purine Nucleotides
DNA
Nucleotides
Cells
Mitochondria
Mutation Rate
Aldehydes
Tobacco Products
Lipid Peroxidation
Animals
Lipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Translesion synthesis past acrolein-derived DNA adducts by human mitochondrial DNA polymerase γ. / Kasiviswanathan, Rajesh; Minko, Irina; Lloyd, Robert (Stephen); Copeland, William C.

In: Journal of Biological Chemistry, Vol. 288, No. 20, 17.05.2013, p. 14247-14255.

Research output: Contribution to journalArticle

Kasiviswanathan, Rajesh ; Minko, Irina ; Lloyd, Robert (Stephen) ; Copeland, William C. / Translesion synthesis past acrolein-derived DNA adducts by human mitochondrial DNA polymerase γ. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 20. pp. 14247-14255.
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