@article{0712348e6eb04b5baf71cf82ad7f10d9,
title = "Translational model of Zika virus disease in baboons",
abstract = "Zika virus (ZIKV) is an emerging mosquito-borne flavivirus with devastating outcomes seen recently in the Americas due to the association of maternal ZIKV infection with fetal microcephaly and other fetal malformations not previously associated with flavivirus infections. Here, we have developed the olive baboon (Papio anubis) as a nonhuman primate (NHP) translational model for the study of ZIKV pathogenesis and associated disease outcomes to contrast and compare with humans and other major NHPs, such as macaques. Following subcutaneous inoculation of adult male and nonpregnant female baboons, viremia was detected at 3 and 4 days postinfection (dpi) with the concordant presentation of a visible rash and conjunctivitis, similar to human ZIKV infection. Furthermore, virus was detected in the mucosa and cerebrospinal fluid. A robust ZIKV-specific IgM and IgG antibody response was also observed in all the animals. These data show striking similarity between humans and the olive baboon following infection with ZIKV, suggesting our model is a suitable translational NHP model to study ZIKV pathogenesis and potential therapeutics.",
keywords = "Baboon, Flavivirus, Nonhuman primate, West Nile virus, ZIKV, Zika virus",
author = "Sunam Gurung and Preno, {Alisha N.} and Dubaut, {Jamie P.} and Hugh Nadeau and Kimberly Hyatt and Nicole Reuter and Bharti Nehete and Wolf, {Roman F.} and Pramod Nehete and Dittmer, {Dirk P.} and Myers, {Dean A.} and Papin, {James F.}",
note = "Funding Information: We thank the Oklahoma Baboon Research Resource for providing baboons; James J. Tomasek, Vice President for Research, University of Oklahoma Health Sciences Center, for support of the project; and Helen Lazear, University of North Carolina Chapel Hill, for providing ZIKV stocks. This study was supported by NIH R21 AI129560, NIH R21 NS103772, NIH P40 OD010988, and funds from the University of Oklahoma Health Sciences Center Vice President for Research. J.F.P., D.A.M., and S.G. designed the experiments, analyzed the data, and drafted the manuscript. S.G. developed and performed viral load assays, ZIKV IgM ELISAs, and cytokine assays. A.N.P., J.F.P, R.F.W. and J.P.D. performed the baboon necropsies and helped with sample collection. N.R. coordinated baboon tissue sampling for processing and performed blood chemistries, CBC panel, and ZIKV and WNV IgG ELISAs. H.N. and K.H. performed the PRNTs. B.N. and P.N. performed the IFN-β ELISPOT assays, and D.P.D. assisted with viral load assays, data analysis, and drafting the manuscript. We declare that we have no competing financial interests. Publisher Copyright: {\textcopyright} 2018 American Society for Microbiology.",
year = "2018",
month = aug,
day = "1",
doi = "10.1128/JVI.00186-18",
language = "English (US)",
volume = "92",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "16",
}