Transforming growth factor (TGF)-β stimulates hepatic jun-B and fos-B proto-oncogenes and decreases albumin mRNA

R. D. Beauchamp, H. M. Sheng, J. Ishizuka, C. M. Townsend, J. C. Thompson, S. F. Lowry, Dana Andersen

Research output: Contribution to journalArticle

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Abstract

Transforming growth factor-β (TGF-β) modulates some components of the acute phase response in hepatic cells. The mechanisms for these actions of TGF-β are largely unknown. The authors recently found that the decrease in albumin mRNA after TGF-β1 treatment required de novo RNA and protein synthesis, suggesting that TGF-β acts through induction of another gene. The purpose of the current study was to determine whether TGF-β1 could regulate the expression of both the jun and fos genes that encode transcriptional regulatory proteins that constitute the AP-1 complex, and to determine whether expression of these genes may be coordinated with the decrease in albumin mRNA. Northern blot hybridization was used to determine levels of specific mRNAs. Transforming growth factor-β1 increased the levels of both jun-B and fos-B mRNA by 60 minutes after treatment of mouse hepatoma (BWTG3) cells. When TGF-β1 was removed from the media after 4 hours, there was a sustained effect of increased jun-B and decreased albumin mRNA (>48 hours), and the subsequent decrease in jun-B levels coincided with the increase in albumin mRNA. The tumor-promoting phorbol ester (phorbol 12-myristate 13- acetate [PMA]), known to induce jun and fos gene expression, caused increases in jun-B and fos-B that preceded the decrease in albumin mRNA levels at 24 hours. These observations are consistent with our hypothesis that jun-B and fos-B induction may participate in downregulation of albumin synthesis as well as other hepatic responses to TGF-β.

Original languageEnglish (US)
Pages (from-to)300-308
Number of pages9
JournalAnnals of Surgery
Volume216
Issue number3
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Proto-Oncogenes
Transforming Growth Factors
Albumins
Messenger RNA
Liver
jun Genes
fos Genes
Gene Expression
Acute-Phase Reaction
Transcription Factor AP-1
Phorbol Esters
Northern Blotting
Hepatocytes
Hepatocellular Carcinoma
Proteins
Acetates
Down-Regulation
RNA

ASJC Scopus subject areas

  • Surgery

Cite this

Transforming growth factor (TGF)-β stimulates hepatic jun-B and fos-B proto-oncogenes and decreases albumin mRNA. / Beauchamp, R. D.; Sheng, H. M.; Ishizuka, J.; Townsend, C. M.; Thompson, J. C.; Lowry, S. F.; Andersen, Dana.

In: Annals of Surgery, Vol. 216, No. 3, 01.01.1992, p. 300-308.

Research output: Contribution to journalArticle

Beauchamp, R. D. ; Sheng, H. M. ; Ishizuka, J. ; Townsend, C. M. ; Thompson, J. C. ; Lowry, S. F. ; Andersen, Dana. / Transforming growth factor (TGF)-β stimulates hepatic jun-B and fos-B proto-oncogenes and decreases albumin mRNA. In: Annals of Surgery. 1992 ; Vol. 216, No. 3. pp. 300-308.
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