TY - JOUR
T1 - Transformation of DNA repair-deficient human diploid fibroblasts with a simian virus 40 plasmid
AU - Wood, C. M.
AU - Timme, T. L.
AU - Hurt, M. M.
AU - Brinkley, B. R.
AU - Ledbetter, D. H.
AU - Moses, R. E.
N1 - Funding Information:
We thank Dr J. Butel for T-antigen detection and helpful comments, and S. A. Ledbetter for expert technical advice. This work was supported by USPHS grants CA41424 and CA37860. During parts of this study, C. M. W. was a Cell Biology Trainee under NIH Training Grant GMl3306, and T. L. T. was a Medical Genetics Trainee under NIH Training Grant GM07526.
PY - 1987/4
Y1 - 1987/4
N2 - Fibroblasts from patients with xeroderma pigmentosum (XP) complementation groups A, C, D, E, and G, as well as Bloom syndrome (BS) and Fanconi anemia (FA) have been transfected with a plasmid, pSV7, containing the early region of Simian virus 40 (SV40). All of the cultures exhibited cytologic changes characteristic of transformed cells and expressed T-antigen. They also contained integrated copies of DNA derived from the vector, and in several cases, extrachromosomally replicated DNA. Not all of the transfected cultures became immortalized. The transformed xeroderma pigmentosum (XP) cultures retained their UV-sensitive phenotype in all but one case. The BS and FA cell lines retained their characteristic phenotype. All of the cultures, except the BS cells, can be readily transfected with the plasmids, pSV2neo and pSV2gpt.
AB - Fibroblasts from patients with xeroderma pigmentosum (XP) complementation groups A, C, D, E, and G, as well as Bloom syndrome (BS) and Fanconi anemia (FA) have been transfected with a plasmid, pSV7, containing the early region of Simian virus 40 (SV40). All of the cultures exhibited cytologic changes characteristic of transformed cells and expressed T-antigen. They also contained integrated copies of DNA derived from the vector, and in several cases, extrachromosomally replicated DNA. Not all of the transfected cultures became immortalized. The transformed xeroderma pigmentosum (XP) cultures retained their UV-sensitive phenotype in all but one case. The BS and FA cell lines retained their characteristic phenotype. All of the cultures, except the BS cells, can be readily transfected with the plasmids, pSV2neo and pSV2gpt.
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U2 - 10.1016/0014-4827(87)90214-X
DO - 10.1016/0014-4827(87)90214-X
M3 - Article
C2 - 3030788
AN - SCOPUS:0023107395
SN - 0014-4827
VL - 169
SP - 543
EP - 553
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -