Transcriptome-wide discovery of microRNA binding sites in Human Brain

Ryan L. Boudreau, Peng Jiang, Brian L. Gilmore, Ryan M. Spengler, Rebecca Tirabassi, Jay Nelson, Christopher A. Ross, Yi Xing, Beverly L. Davidson

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The orchestration of brain function requires complex gene regulatory networks that are modulated, in part, by microRNAs (miRNAs). These noncoding RNAs associate with argonaute (Ago) proteins in order to direct posttranscriptional gene suppression via base pairing with target transcripts. In order to better understand how miRNAs contribute to human-specialized brain processes and neurological phenotypes, identifying their targets is of paramount importance. Here, we address the latter by profiling Ago2:RNA interactions using HITS-CLIP to generate a transcriptome-wide map of miRNA binding sites in human brain. We uncovered ~7,000 stringent Ago2 binding sites that are highly enriched for conserved sequences corresponding to abundant brain miRNAs. This interactome points to functional miRNA:target pairs across >3,000 genes and represents a valuable resource for accelerating our understanding of miRNA functions in brain. We demonstrate the utility of this map for exploring clinically relevant miRNA binding sites that may facilitate the translation of genetic studies of complex neuropsychiatric diseases into therapeutics.

Original languageEnglish (US)
Pages (from-to)294-305
Number of pages12
JournalNeuron
Volume81
Issue number2
DOIs
StatePublished - Jan 22 2014

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MicroRNAs
Transcriptome
Binding Sites
Brain
Argonaute Proteins
Untranslated RNA
Conserved Sequence
Gene Regulatory Networks
Protein Biosynthesis
Base Pairing
Genes
RNA
Phenotype

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Boudreau, R. L., Jiang, P., Gilmore, B. L., Spengler, R. M., Tirabassi, R., Nelson, J., ... Davidson, B. L. (2014). Transcriptome-wide discovery of microRNA binding sites in Human Brain. Neuron, 81(2), 294-305. https://doi.org/10.1016/j.neuron.2013.10.062

Transcriptome-wide discovery of microRNA binding sites in Human Brain. / Boudreau, Ryan L.; Jiang, Peng; Gilmore, Brian L.; Spengler, Ryan M.; Tirabassi, Rebecca; Nelson, Jay; Ross, Christopher A.; Xing, Yi; Davidson, Beverly L.

In: Neuron, Vol. 81, No. 2, 22.01.2014, p. 294-305.

Research output: Contribution to journalArticle

Boudreau, RL, Jiang, P, Gilmore, BL, Spengler, RM, Tirabassi, R, Nelson, J, Ross, CA, Xing, Y & Davidson, BL 2014, 'Transcriptome-wide discovery of microRNA binding sites in Human Brain', Neuron, vol. 81, no. 2, pp. 294-305. https://doi.org/10.1016/j.neuron.2013.10.062
Boudreau RL, Jiang P, Gilmore BL, Spengler RM, Tirabassi R, Nelson J et al. Transcriptome-wide discovery of microRNA binding sites in Human Brain. Neuron. 2014 Jan 22;81(2):294-305. https://doi.org/10.1016/j.neuron.2013.10.062
Boudreau, Ryan L. ; Jiang, Peng ; Gilmore, Brian L. ; Spengler, Ryan M. ; Tirabassi, Rebecca ; Nelson, Jay ; Ross, Christopher A. ; Xing, Yi ; Davidson, Beverly L. / Transcriptome-wide discovery of microRNA binding sites in Human Brain. In: Neuron. 2014 ; Vol. 81, No. 2. pp. 294-305.
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