Transcriptome profiling reveals disruption of innate immunity in chronic heavy ethanol consuming female rhesus macaques

Suhas Sureshchandra, Maham Rais, Cara Stull, Kathleen (Kathy) Grant, Ilhem Messaoudi

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    It is well established that heavy ethanol consumption interferes with the immune system and inflammatory processes, resulting in increased risk for infectious and chronic diseases. However, these processes have yet to be systematically studied in a dose and sex-dependent manner. In this study, we investigated the impact of chronic heavy ethanol consumption on gene expression using RNA-seq in peripheral blood mononuclear cells isolated from female rhesus macaques with daily consumption of 4% ethanol available 22hr/day for 12 months resulting in average ethanol consumption of 4.3 g/kg/day (considered heavy drinking). Differential gene expression analysis was performed using edgeR and gene enrichment analysis using MetaCore™. We identified 1106 differentially expressed genes, meeting the criterion of ≥ two-fold change and p-value ≤ 0.05 in expression (445 up- and 661 down-regulated). Pathway analysis of the 879 genes with characterized identifiers showed that the most enriched gene ontology processes were "response to wounding", "blood coagulation", "immune system process", and "regulation of signaling". Changes in gene expression were seen despite the lack of differences in the frequency of any major immune cell subtype between ethanol and controls, suggesting that heavy ethanol consumption modulates gene expression at the cellular level rather than altering the distribution of peripheral blood mononuclear cells. Collectively, these observations provide mechanisms to explain the higher incidence of infection, delay in wound healing, and increase in cardiovascular disease seen in subjects with Alcohol use disorder.

    Original languageEnglish (US)
    Article numbere0159295
    JournalPLoS One
    Volume11
    Issue number7
    DOIs
    StatePublished - Jul 1 2016

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    Gene Expression Profiling
    Macaca mulatta
    transcriptomics
    Innate Immunity
    Ethanol
    ethanol
    Gene expression
    Immune System Phenomena
    Genes
    Gene Expression
    gene expression
    Blood
    Immune system
    mononuclear leukocytes
    immune system
    Blood Cells
    genes
    blood coagulation
    Gene Ontology
    Blood Coagulation

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Transcriptome profiling reveals disruption of innate immunity in chronic heavy ethanol consuming female rhesus macaques. / Sureshchandra, Suhas; Rais, Maham; Stull, Cara; Grant, Kathleen (Kathy); Messaoudi, Ilhem.

    In: PLoS One, Vol. 11, No. 7, e0159295, 01.07.2016.

    Research output: Contribution to journalArticle

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