Transcriptome analysis of the murine forelimb and hindlimb autopod

Siming Shou, Virginia Scott, Cheryl Reed, Robert Hitzemann, H. Scott Stadler

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE-430). Forty-four transcripts with expression differences higher than 2-fold (T test, P ≤ 0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10, Frzb, Tbx18, and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb-signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change >2, P ≤ 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore- and hindlimb-specific clusters (P ≤ 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type.

Original languageEnglish (US)
Pages (from-to)74-89
Number of pages16
JournalDevelopmental Dynamics
Volume234
Issue number1
DOIs
StatePublished - Sep 1 2005

Keywords

  • Distal limb development
  • Gene expression
  • Microarray
  • Transcriptome

ASJC Scopus subject areas

  • Developmental Biology

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