Transcriptome analysis of the murine forelimb and hindlimb autopod

Siming Shou, Virginia Scott, Cheryl Reed, Robert Hitzemann, H. Scott Stadler

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

To gain insight into the coordination of gene expression profiles during forelimb and hindlimb differentiation, a transcriptome analysis of mouse embryonic autopod tissues was performed using Affymetrix Murine Gene Chips (MOE-430). Forty-four transcripts with expression differences higher than 2-fold (T test, P ≤ 0.05) were detected between forelimb and hindlimb tissues including 38 new transcripts such as Rdh10, Frzb, Tbx18, and Hip that exhibit differential limb expression. A comparison of gene expression profiles in the forelimb, hindlimb, and brain revealed 24 limb-signature genes whose expression was significantly enriched in limb autopod versus brain tissue (fold change >2, P ≤ 0.05). Interestingly, the genes exhibiting enrichment in the developing autopod also segregated into significant fore- and hindlimb-specific clusters (P ≤ 0.05) suggesting that by E 12.5, unique gene combinations are being used during the differentiation of each autopod type.

Original languageEnglish (US)
Pages (from-to)74-89
Number of pages16
JournalDevelopmental Dynamics
Volume234
Issue number1
DOIs
StatePublished - Sep 2005

Fingerprint

Forelimb
Gene Expression Profiling
Hindlimb
Transcriptome
Extremities
Tocopherols
Brain
Oligonucleotide Array Sequence Analysis
Genes
Hip

Keywords

  • Distal limb development
  • Gene expression
  • Microarray
  • Transcriptome

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Transcriptome analysis of the murine forelimb and hindlimb autopod. / Shou, Siming; Scott, Virginia; Reed, Cheryl; Hitzemann, Robert; Stadler, H. Scott.

In: Developmental Dynamics, Vol. 234, No. 1, 09.2005, p. 74-89.

Research output: Contribution to journalArticle

Shou, Siming ; Scott, Virginia ; Reed, Cheryl ; Hitzemann, Robert ; Stadler, H. Scott. / Transcriptome analysis of the murine forelimb and hindlimb autopod. In: Developmental Dynamics. 2005 ; Vol. 234, No. 1. pp. 74-89.
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