Transcriptional responses to tubule challenges

Steven R. Gullans, David Cohen, Ryoji Kojima, Jeffrey Randall, Barry M. Brenner, Bento Santos, Alejandro Chevaile

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Adaptation to physiological stimuli often involves changes in gene transcription. Studies of hyperosmolar stress in renal epithelial cells have provided an ideal paradigm for understanding regulation of gene expression. Renal epithelial cells respond very differently to hyperosmolar NaCl and urea and several strategies including cloning based on known biological function, candidate gene analysis, and differential display analysis have successfully identified many genes induced by these hyperosmolar challenges. Hyperosmolar NaCl produces adverse effects on cellular biosynthetic processes and compensatory increases are observed in transcription of transporters, stress proteins, and metabolic enxymes. In contrast, hyperosmolar urea fails to inhibit biosynthetic processes but, nonetheless, initiates a very specific program of gene expression in renal epithelial cells. This program appears to involve a urea sensor/receptor system which activates transcription and translation of the zinc-finger transcription factor Egr-1. This work highlights the concept that rapid analysis of differential gene expression will enable one to define cellular programs of gene expression involving up- and down-regulation of functionally-related gene families.

Original languageEnglish (US)
Pages (from-to)1678-1681
Number of pages4
JournalKidney International
Volume49
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

Urea
Epithelial Cells
Kidney
Gene Expression
Genes
Physiological Adaptation
Zinc Fingers
Gene Expression Regulation
Genetic Association Studies
Heat-Shock Proteins
Organism Cloning
Transcription Factors
Up-Regulation
Down-Regulation

ASJC Scopus subject areas

  • Nephrology

Cite this

Gullans, S. R., Cohen, D., Kojima, R., Randall, J., Brenner, B. M., Santos, B., & Chevaile, A. (1996). Transcriptional responses to tubule challenges. Kidney International, 49(6), 1678-1681.

Transcriptional responses to tubule challenges. / Gullans, Steven R.; Cohen, David; Kojima, Ryoji; Randall, Jeffrey; Brenner, Barry M.; Santos, Bento; Chevaile, Alejandro.

In: Kidney International, Vol. 49, No. 6, 1996, p. 1678-1681.

Research output: Contribution to journalArticle

Gullans, SR, Cohen, D, Kojima, R, Randall, J, Brenner, BM, Santos, B & Chevaile, A 1996, 'Transcriptional responses to tubule challenges', Kidney International, vol. 49, no. 6, pp. 1678-1681.
Gullans SR, Cohen D, Kojima R, Randall J, Brenner BM, Santos B et al. Transcriptional responses to tubule challenges. Kidney International. 1996;49(6):1678-1681.
Gullans, Steven R. ; Cohen, David ; Kojima, Ryoji ; Randall, Jeffrey ; Brenner, Barry M. ; Santos, Bento ; Chevaile, Alejandro. / Transcriptional responses to tubule challenges. In: Kidney International. 1996 ; Vol. 49, No. 6. pp. 1678-1681.
@article{bb7e4f7c406f46b2acc93adbcbf9c903,
title = "Transcriptional responses to tubule challenges",
abstract = "Adaptation to physiological stimuli often involves changes in gene transcription. Studies of hyperosmolar stress in renal epithelial cells have provided an ideal paradigm for understanding regulation of gene expression. Renal epithelial cells respond very differently to hyperosmolar NaCl and urea and several strategies including cloning based on known biological function, candidate gene analysis, and differential display analysis have successfully identified many genes induced by these hyperosmolar challenges. Hyperosmolar NaCl produces adverse effects on cellular biosynthetic processes and compensatory increases are observed in transcription of transporters, stress proteins, and metabolic enxymes. In contrast, hyperosmolar urea fails to inhibit biosynthetic processes but, nonetheless, initiates a very specific program of gene expression in renal epithelial cells. This program appears to involve a urea sensor/receptor system which activates transcription and translation of the zinc-finger transcription factor Egr-1. This work highlights the concept that rapid analysis of differential gene expression will enable one to define cellular programs of gene expression involving up- and down-regulation of functionally-related gene families.",
author = "Gullans, {Steven R.} and David Cohen and Ryoji Kojima and Jeffrey Randall and Brenner, {Barry M.} and Bento Santos and Alejandro Chevaile",
year = "1996",
language = "English (US)",
volume = "49",
pages = "1678--1681",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - Transcriptional responses to tubule challenges

AU - Gullans, Steven R.

AU - Cohen, David

AU - Kojima, Ryoji

AU - Randall, Jeffrey

AU - Brenner, Barry M.

AU - Santos, Bento

AU - Chevaile, Alejandro

PY - 1996

Y1 - 1996

N2 - Adaptation to physiological stimuli often involves changes in gene transcription. Studies of hyperosmolar stress in renal epithelial cells have provided an ideal paradigm for understanding regulation of gene expression. Renal epithelial cells respond very differently to hyperosmolar NaCl and urea and several strategies including cloning based on known biological function, candidate gene analysis, and differential display analysis have successfully identified many genes induced by these hyperosmolar challenges. Hyperosmolar NaCl produces adverse effects on cellular biosynthetic processes and compensatory increases are observed in transcription of transporters, stress proteins, and metabolic enxymes. In contrast, hyperosmolar urea fails to inhibit biosynthetic processes but, nonetheless, initiates a very specific program of gene expression in renal epithelial cells. This program appears to involve a urea sensor/receptor system which activates transcription and translation of the zinc-finger transcription factor Egr-1. This work highlights the concept that rapid analysis of differential gene expression will enable one to define cellular programs of gene expression involving up- and down-regulation of functionally-related gene families.

AB - Adaptation to physiological stimuli often involves changes in gene transcription. Studies of hyperosmolar stress in renal epithelial cells have provided an ideal paradigm for understanding regulation of gene expression. Renal epithelial cells respond very differently to hyperosmolar NaCl and urea and several strategies including cloning based on known biological function, candidate gene analysis, and differential display analysis have successfully identified many genes induced by these hyperosmolar challenges. Hyperosmolar NaCl produces adverse effects on cellular biosynthetic processes and compensatory increases are observed in transcription of transporters, stress proteins, and metabolic enxymes. In contrast, hyperosmolar urea fails to inhibit biosynthetic processes but, nonetheless, initiates a very specific program of gene expression in renal epithelial cells. This program appears to involve a urea sensor/receptor system which activates transcription and translation of the zinc-finger transcription factor Egr-1. This work highlights the concept that rapid analysis of differential gene expression will enable one to define cellular programs of gene expression involving up- and down-regulation of functionally-related gene families.

UR - http://www.scopus.com/inward/record.url?scp=0029889086&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029889086&partnerID=8YFLogxK

M3 - Article

C2 - 8743475

AN - SCOPUS:0029889086

VL - 49

SP - 1678

EP - 1681

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 6

ER -