Transcriptional profiling of rhesus monkey embryonic stem cells

James A. Byrne, Shoukhrat Mitalipov, Lisa Clepper, Don P. Wolf

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Embryonic stem cells (ESCs) may be able to cure or alleviate the symptoms of various degenerative diseases. However, unresolved issues regarding survival, functionality, and tumor formation mean a prudent approach should be adopted towards advancing ESCs into human clinical trials. The rhesus monkey provides an ideal model organism for developing strategies to prevent immune rejection and test the feasibility, safety, and efficacy of ESC-based medical treatments. Transcriptional profiling of rhesus monkey ESCs provides a foundation for preclinical ESC research in this species. In the present study, we used microarray technology, immunocytochemistry, reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) to characterize and transcriptionally profile rhesus monkey ESCs. We identified 367 stemness gene candidates that were highly (>85%) conserved across five different ESC lines. Rhesus monkey ESC lines maintained a pluripotent undifferentiated state over a wide range of POU5F1 (also known as OCT4) expression levels, and comparisons between rhesus monkey, mouse, and human stemness genes revealed five mammalian stemness genes: CCNB1, GDF3, LEFTB, POU5F1, and NANOG. These five mammalian genes are strongly expressed in rhesus monkey, mouse, and human ESCs, albeit only in the undifferentiated state, and represent the core key mammalian stemness factors.

    Original languageEnglish (US)
    Pages (from-to)908-915
    Number of pages8
    JournalBiology of Reproduction
    Volume75
    Issue number6
    DOIs
    StatePublished - Dec 2006

    Fingerprint

    Embryonic Stem Cells
    Macaca mulatta
    Genes
    Stem Cell Research
    Cell Line
    Reverse Transcription
    Real-Time Polymerase Chain Reaction
    Immunohistochemistry
    Clinical Trials
    Technology
    Safety
    Polymerase Chain Reaction
    Survival
    Neoplasms

    Keywords

    • Embryo
    • Gene regulation
    • Microarray
    • Rhesus
    • Stem cells

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Transcriptional profiling of rhesus monkey embryonic stem cells. / Byrne, James A.; Mitalipov, Shoukhrat; Clepper, Lisa; Wolf, Don P.

    In: Biology of Reproduction, Vol. 75, No. 6, 12.2006, p. 908-915.

    Research output: Contribution to journalArticle

    Byrne, James A. ; Mitalipov, Shoukhrat ; Clepper, Lisa ; Wolf, Don P. / Transcriptional profiling of rhesus monkey embryonic stem cells. In: Biology of Reproduction. 2006 ; Vol. 75, No. 6. pp. 908-915.
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