Trajectory analysis unveils Reelin’s role in the directed migration of granule cells in the dentate gyrus

Shaobo Wang, Bianka Brunne, Shanting Zhao, Xuejun Chai, Jiawei Li, Jeremie Lau, Antonio Virgilio Failla, Bernd Zobiak, Mirjam Sibbe, Gary Westbrook, David Lutz, Michael Frotscher

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Reelin controls neuronal migration and layer formation. Previous studies in reeler mice deficient in Reelin focused on the result of the developmental process in fixed tissue sections. It has remained unclear whether Reelin affects the migratory process, migration directionality, or migrating neurons guided by the radial glial scaffold. Moreover, Reelin has been regarded as an attractive signal because newly generated neurons migrate toward the Reelin-containing marginal zone. Conversely, Reelin might be a stop signal because migrating neurons in reeler, but not in wild-type mice, invade the marginal zone. Here, we monitored the migration of newly generated proopiomelanocortin-EGFP-expressing dentate granule cells in slice cultures from reeler, reeler-like mutants and wild-type mice of either sex using real-time microscopy. We discovered that not the actual migratory process and migratory speed, but migration directionality of the granule cells is controlled by Reelin. While wild-type granule cells migrated toward the marginal zone of the dentate gyrus, neurons in cultures from reeler and reeler-like mutants migrated randomly in all directions as revealed by vector analyses of migratory trajectories. Moreover, live imaging of granule cells in reeler slices cocultured to wild-type dentate gyrus showed that the reeler neurons changed their directions and migrated toward the Reelin-containing marginal zone of the wild-type culture, thus forming a compact granule cell layer. In contrast, directed migration was not observed when Reelin was ubiquitously present in the medium of reeler slices. These results indicate that topographically administered Reelin controls the formation of a granule cell layer.

Original languageEnglish (US)
Pages (from-to)137-148
Number of pages12
JournalJournal of Neuroscience
Volume38
Issue number1
DOIs
StatePublished - Jan 3 2018

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Dentate Gyrus
Cell Movement
Neurons
Neurologic Mutant Mice
Pro-Opiomelanocortin
Neuroglia
Microscopy

Keywords

  • Dab1 phosphorylation
  • Hippocampus
  • Live imaging
  • Neuronal migration
  • Reeler-like
  • Reelin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Trajectory analysis unveils Reelin’s role in the directed migration of granule cells in the dentate gyrus. / Wang, Shaobo; Brunne, Bianka; Zhao, Shanting; Chai, Xuejun; Li, Jiawei; Lau, Jeremie; Failla, Antonio Virgilio; Zobiak, Bernd; Sibbe, Mirjam; Westbrook, Gary; Lutz, David; Frotscher, Michael.

In: Journal of Neuroscience, Vol. 38, No. 1, 03.01.2018, p. 137-148.

Research output: Contribution to journalArticle

Wang, S, Brunne, B, Zhao, S, Chai, X, Li, J, Lau, J, Failla, AV, Zobiak, B, Sibbe, M, Westbrook, G, Lutz, D & Frotscher, M 2018, 'Trajectory analysis unveils Reelin’s role in the directed migration of granule cells in the dentate gyrus', Journal of Neuroscience, vol. 38, no. 1, pp. 137-148. https://doi.org/10.1523/JNEUROSCI.0988-17.2017
Wang, Shaobo ; Brunne, Bianka ; Zhao, Shanting ; Chai, Xuejun ; Li, Jiawei ; Lau, Jeremie ; Failla, Antonio Virgilio ; Zobiak, Bernd ; Sibbe, Mirjam ; Westbrook, Gary ; Lutz, David ; Frotscher, Michael. / Trajectory analysis unveils Reelin’s role in the directed migration of granule cells in the dentate gyrus. In: Journal of Neuroscience. 2018 ; Vol. 38, No. 1. pp. 137-148.
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