Trafficking of aminoglycosides into endolymph in vivo

Qi Wang, Peter Steyger

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In vitro, aminoglycosides increase the stiffness of cochlear hair cell stereocilia, altering bundle motion and transduction kinetics. Aminoglycosides also permeate the mechanosensitive transduction channel and rapidly initiate cytotoxicity in hair cells. If these effects occur in vivo, aminoglycosides would need to enter endolymph. The most direct route for systemically-administered aminoglycosides to enter endolymph is by trafficking from strial capillaries across the stria vascularis. An as-yet-unidentified active transporter is required to translocate aminoglycosides from the intra-strial space into the cytoplasm of marginal cells. Once in marginal cells, aminoglycosides would passively flow down the electrochemical gradient into endolymph. We present data that support a trans-strial trafficking route of aminoglycosides into endolymph, where they can then interfere with the mechanosensitive hair bundles.

Original languageEnglish (US)
Title of host publicationConcepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008
EditorsDavid T. Kemp, Nigel P. Cooper
PublisherWorld Scientific Publishing Co. Pte Ltd
Pages439-443
Number of pages5
ISBN (Electronic)9789812833778
StatePublished - Jan 1 2008
Event10th International Workshop on the Mechanics of Hearing - Concepts and Challenges in the Biophysics of Hearing, MoH 2008 - Newcastle, United Kingdom
Duration: Jul 27 2008Jul 31 2008

Publication series

NameConcepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008

Conference

Conference10th International Workshop on the Mechanics of Hearing - Concepts and Challenges in the Biophysics of Hearing, MoH 2008
CountryUnited Kingdom
CityNewcastle
Period7/27/087/31/08

Fingerprint

Endolymph
Aminoglycosides
Auditory Hair Cells
Cells
Stereocilia
Stria Vascularis
Cytotoxicity
Cytoplasm
Stiffness
Kinetics

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering

Cite this

Wang, Q., & Steyger, P. (2008). Trafficking of aminoglycosides into endolymph in vivo. In D. T. Kemp, & N. P. Cooper (Eds.), Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008 (pp. 439-443). (Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008). World Scientific Publishing Co. Pte Ltd.

Trafficking of aminoglycosides into endolymph in vivo. / Wang, Qi; Steyger, Peter.

Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008. ed. / David T. Kemp; Nigel P. Cooper. World Scientific Publishing Co. Pte Ltd, 2008. p. 439-443 (Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, Q & Steyger, P 2008, Trafficking of aminoglycosides into endolymph in vivo. in DT Kemp & NP Cooper (eds), Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008. Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008, World Scientific Publishing Co. Pte Ltd, pp. 439-443, 10th International Workshop on the Mechanics of Hearing - Concepts and Challenges in the Biophysics of Hearing, MoH 2008, Newcastle, United Kingdom, 7/27/08.
Wang Q, Steyger P. Trafficking of aminoglycosides into endolymph in vivo. In Kemp DT, Cooper NP, editors, Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008. World Scientific Publishing Co. Pte Ltd. 2008. p. 439-443. (Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008).
Wang, Qi ; Steyger, Peter. / Trafficking of aminoglycosides into endolymph in vivo. Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008. editor / David T. Kemp ; Nigel P. Cooper. World Scientific Publishing Co. Pte Ltd, 2008. pp. 439-443 (Concepts and Challenges in the Biophysics of Hearing - Proceedings of the 10th International Workshop on the Mechanics of Hearing, MoH 2008).
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