Tractable Cre-lox system for stochastic alteration of genes in mice

Ashleigh J. Miller; Sandra D. Dudley; Jen Lan Tsao; Darryl Shibata; R. Michael Liskay

doi:10.1038/nmeth.1183

Tractable Cre-lox system for stochastic alteration of genes in mice

Ashleigh J. Miller, Sandra D. Dudley, Jen Lan Tsao, Darryl Shibata, R. Michael Liskay

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.

Original language	English (US)
Pages (from-to)	227-229
Number of pages	3
Journal	Nature Methods
Volume	5
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.1183
State	Published - Mar 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.1183

Cite this

@article{22664ff9fddd4257b0db22c6c28fa866,

title = "Tractable Cre-lox system for stochastic alteration of genes in mice",

abstract = "We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.",

author = "Miller, {Ashleigh J.} and Dudley, {Sandra D.} and Tsao, {Jen Lan} and Darryl Shibata and Liskay, {R. Michael}",

note = "Funding Information: We thank M. Wong, N. Erdeniz, J. Johnson, O. Reilly and K. MacDonald for critically reading the manuscript. This work was supported by US National Institutes of Health grants R37 GM32741 to R.M.L. and 2 RO1 CA 80077 to D.S.",

year = "2008",

month = mar,

doi = "10.1038/nmeth.1183",

language = "English (US)",

volume = "5",

pages = "227--229",

journal = "PLoS Medicine",

issn = "1549-1277",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - Tractable Cre-lox system for stochastic alteration of genes in mice

AU - Miller, Ashleigh J.

AU - Dudley, Sandra D.

AU - Tsao, Jen Lan

AU - Shibata, Darryl

AU - Liskay, R. Michael

N1 - Funding Information: We thank M. Wong, N. Erdeniz, J. Johnson, O. Reilly and K. MacDonald for critically reading the manuscript. This work was supported by US National Institutes of Health grants R37 GM32741 to R.M.L. and 2 RO1 CA 80077 to D.S.

PY - 2008/3

Y1 - 2008/3

N2 - We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.

AB - We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.

UR - http://www.scopus.com/inward/record.url?scp=40149101401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40149101401&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1183

DO - 10.1038/nmeth.1183

M3 - Article

C2 - 18264106

AN - SCOPUS:40149101401

SN - 1549-1277

VL - 5

SP - 227

EP - 229

JO - PLoS Medicine

JF - PLoS Medicine

IS - 3

ER -

Tractable Cre-lox system for stochastic alteration of genes in mice

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this