Tractable Cre-lox system for stochastic alteration of genes in mice

Ashleigh J. Miller, Sandra D. Dudley, Jen Lan Tsao, Darryl Shibata, R. Michael Liskay

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.

Original languageEnglish (US)
Pages (from-to)227-229
Number of pages3
JournalNature Methods
Volume5
Issue number3
DOIs
StatePublished - Mar 2008

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Miller, A. J., Dudley, S. D., Tsao, J. L., Shibata, D., & Liskay, R. M. (2008). Tractable Cre-lox system for stochastic alteration of genes in mice. Nature Methods, 5(3), 227-229. https://doi.org/10.1038/nmeth.1183