Tractable Cre-lox system for stochastic alteration of genes in mice

Ashleigh J. Miller, Sandra D. Dudley, Jen Lan Tsao, Darryl Shibata, Robert (Mike) Liskay

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.

Original languageEnglish (US)
Pages (from-to)227-229
Number of pages3
JournalNature Methods
Volume5
Issue number3
DOIs
StatePublished - Mar 2008

Fingerprint

Repair
Genes
Aging of materials
Chemical activation
Cells
Tissue
DNA Mismatch Repair
DNA
Cell Division
Genetic Recombination
Neoplasms
Mutation

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Miller, A. J., Dudley, S. D., Tsao, J. L., Shibata, D., & Liskay, R. M. (2008). Tractable Cre-lox system for stochastic alteration of genes in mice. Nature Methods, 5(3), 227-229. https://doi.org/10.1038/nmeth.1183

Tractable Cre-lox system for stochastic alteration of genes in mice. / Miller, Ashleigh J.; Dudley, Sandra D.; Tsao, Jen Lan; Shibata, Darryl; Liskay, Robert (Mike).

In: Nature Methods, Vol. 5, No. 3, 03.2008, p. 227-229.

Research output: Contribution to journalArticle

Miller, AJ, Dudley, SD, Tsao, JL, Shibata, D & Liskay, RM 2008, 'Tractable Cre-lox system for stochastic alteration of genes in mice', Nature Methods, vol. 5, no. 3, pp. 227-229. https://doi.org/10.1038/nmeth.1183
Miller AJ, Dudley SD, Tsao JL, Shibata D, Liskay RM. Tractable Cre-lox system for stochastic alteration of genes in mice. Nature Methods. 2008 Mar;5(3):227-229. https://doi.org/10.1038/nmeth.1183
Miller, Ashleigh J. ; Dudley, Sandra D. ; Tsao, Jen Lan ; Shibata, Darryl ; Liskay, Robert (Mike). / Tractable Cre-lox system for stochastic alteration of genes in mice. In: Nature Methods. 2008 ; Vol. 5, No. 3. pp. 227-229.
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